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Genetic Variation of the Fc Gamma Receptor 3B Gene and Association with Rheumatoid Arthritis
BACKGROUND: Fc gamma receptors (FcγRs) play a crucial role in immunity by linking IgG antibody-mediated responses with cellular effector and regulatory functions. Genetic variants in these receptors have been previously identified as risk factors for several chronic inflammatory conditions. The pres...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950138/ https://www.ncbi.nlm.nih.gov/pubmed/20957197 http://dx.doi.org/10.1371/journal.pone.0013173 |
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author | Marques, Rute B. Thabet, Mohamed M. White, Stefan J. Houwing-Duistermaat, Jeanine J. Bakker, Aleida M. Hendriks, Gert-Jan Zhernakova, Alexandra Huizinga, Tom W. van der Helm-van Mil, Annette H. Toes, Rene E. |
author_facet | Marques, Rute B. Thabet, Mohamed M. White, Stefan J. Houwing-Duistermaat, Jeanine J. Bakker, Aleida M. Hendriks, Gert-Jan Zhernakova, Alexandra Huizinga, Tom W. van der Helm-van Mil, Annette H. Toes, Rene E. |
author_sort | Marques, Rute B. |
collection | PubMed |
description | BACKGROUND: Fc gamma receptors (FcγRs) play a crucial role in immunity by linking IgG antibody-mediated responses with cellular effector and regulatory functions. Genetic variants in these receptors have been previously identified as risk factors for several chronic inflammatory conditions. The present study aimed to investigate the presence of copy number variations (CNVs) in the FCGR3B gene and its potential association with the autoimmune disease rheumatoid arthritis (RA). METHODOLOGY/PRINCIPAL FINDINGS: CNV of the FCGR3B gene was studied using Multiplex Ligation Dependent Probe Amplification (MLPA) in 518 Dutch RA patients and 304 healthy controls. Surprisingly, three independent MLPA probes targeting the FCGR3B promoter measured different CNV frequencies, with probe#1 and #2 measuring 0 to 5 gene copies and probe#3 showing little evidence of CNV. Quantitative-PCR correlated with the copy number results from MLPA probe#2, which detected low copy number (1 copy) in 6.7% and high copy number (≥3 copies) in 9.4% of the control population. No significant difference was observed between RA patients and the healthy controls, neither in the low copy nor the high copy number groups (p-values = 0.36 and 0.71, respectively). Sequencing of the FCGR3B promoter region revealed an insertion/deletion (indel) that explained the disparate CNV results of MLPA probe#1. Finally, a non-significant trend was found between the novel -256A>TG indel and RA (40.7% in healthy controls versus 35.9% in RA patients; P = 0.08). CONCLUSIONS/SIGNIFICANCE: The current study highlights the complexity and poor characterization of the FCGR3B gene sequence, indicating that the design and interpretation of genotyping assays based on specific probe sequences must be performed with caution. Nonetheless, we confirmed the presence of CNV and identified novel polymorphisms in the FCGR3B gene in the Dutch population. Although no association was found between RA and FCGR3B CNV, the possible protective effect of the -256A>TG indel polymorphism must be addressed in larger studies. |
format | Text |
id | pubmed-2950138 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29501382010-10-18 Genetic Variation of the Fc Gamma Receptor 3B Gene and Association with Rheumatoid Arthritis Marques, Rute B. Thabet, Mohamed M. White, Stefan J. Houwing-Duistermaat, Jeanine J. Bakker, Aleida M. Hendriks, Gert-Jan Zhernakova, Alexandra Huizinga, Tom W. van der Helm-van Mil, Annette H. Toes, Rene E. PLoS One Research Article BACKGROUND: Fc gamma receptors (FcγRs) play a crucial role in immunity by linking IgG antibody-mediated responses with cellular effector and regulatory functions. Genetic variants in these receptors have been previously identified as risk factors for several chronic inflammatory conditions. The present study aimed to investigate the presence of copy number variations (CNVs) in the FCGR3B gene and its potential association with the autoimmune disease rheumatoid arthritis (RA). METHODOLOGY/PRINCIPAL FINDINGS: CNV of the FCGR3B gene was studied using Multiplex Ligation Dependent Probe Amplification (MLPA) in 518 Dutch RA patients and 304 healthy controls. Surprisingly, three independent MLPA probes targeting the FCGR3B promoter measured different CNV frequencies, with probe#1 and #2 measuring 0 to 5 gene copies and probe#3 showing little evidence of CNV. Quantitative-PCR correlated with the copy number results from MLPA probe#2, which detected low copy number (1 copy) in 6.7% and high copy number (≥3 copies) in 9.4% of the control population. No significant difference was observed between RA patients and the healthy controls, neither in the low copy nor the high copy number groups (p-values = 0.36 and 0.71, respectively). Sequencing of the FCGR3B promoter region revealed an insertion/deletion (indel) that explained the disparate CNV results of MLPA probe#1. Finally, a non-significant trend was found between the novel -256A>TG indel and RA (40.7% in healthy controls versus 35.9% in RA patients; P = 0.08). CONCLUSIONS/SIGNIFICANCE: The current study highlights the complexity and poor characterization of the FCGR3B gene sequence, indicating that the design and interpretation of genotyping assays based on specific probe sequences must be performed with caution. Nonetheless, we confirmed the presence of CNV and identified novel polymorphisms in the FCGR3B gene in the Dutch population. Although no association was found between RA and FCGR3B CNV, the possible protective effect of the -256A>TG indel polymorphism must be addressed in larger studies. Public Library of Science 2010-10-05 /pmc/articles/PMC2950138/ /pubmed/20957197 http://dx.doi.org/10.1371/journal.pone.0013173 Text en Marques et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Marques, Rute B. Thabet, Mohamed M. White, Stefan J. Houwing-Duistermaat, Jeanine J. Bakker, Aleida M. Hendriks, Gert-Jan Zhernakova, Alexandra Huizinga, Tom W. van der Helm-van Mil, Annette H. Toes, Rene E. Genetic Variation of the Fc Gamma Receptor 3B Gene and Association with Rheumatoid Arthritis |
title | Genetic Variation of the Fc Gamma Receptor 3B Gene and Association with Rheumatoid Arthritis |
title_full | Genetic Variation of the Fc Gamma Receptor 3B Gene and Association with Rheumatoid Arthritis |
title_fullStr | Genetic Variation of the Fc Gamma Receptor 3B Gene and Association with Rheumatoid Arthritis |
title_full_unstemmed | Genetic Variation of the Fc Gamma Receptor 3B Gene and Association with Rheumatoid Arthritis |
title_short | Genetic Variation of the Fc Gamma Receptor 3B Gene and Association with Rheumatoid Arthritis |
title_sort | genetic variation of the fc gamma receptor 3b gene and association with rheumatoid arthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950138/ https://www.ncbi.nlm.nih.gov/pubmed/20957197 http://dx.doi.org/10.1371/journal.pone.0013173 |
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