Chitosanase-based method for RNA isolation from cells transfected with chitosan/siRNA nanocomplexes for real-time RT-PCR in gene silencing

Chitosan, a well known natural cationic polysaccharide, has been successfully implemented in vitro and in vivo as a nonviral delivery system for both plasmid DNA and siRNA. While using chitosan/siRNA polyplexes to knock down specific targets, we have underestimated the effect of nucleic acids bindin...

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Detalles Bibliográficos
Autores principales: Alameh, Mohamad, Jean, Myriam, DeJesus, Diogo, Buschmann, Michael D, Merzouki, Abderrazzak
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2010
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2950405/
https://www.ncbi.nlm.nih.gov/pubmed/20957169

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