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Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger

In this study we successfully constructed a full-length cDNA library from Siberian tiger, Panthera tigris altaica, the most well-known wild Animal. Total RNA was extracted from cultured Siberian tiger fibroblasts in vitro. The titers of primary and amplified libraries were 1.30×10(6) pfu/ml and 1.62...

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Detalles Bibliográficos
Autores principales:	Liu, Chang-Qing, Lu, Tao-Feng, Feng, Bao-Gang, Liu, Dan, Guan, Wei-Jun, Ma, Yue-Hui
Formato:	Texto
Lenguaje:	English
Publicado:	Ivyspring International Publisher 2010
Materias:	Short Research Communication
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2952408/ https://www.ncbi.nlm.nih.gov/pubmed/20941376

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