Human BRCA2 protein promotes RAD51 filament formation on RPA-covered ssDNA

BRCA2 is an important tumor suppressor and functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on ssDNA to perform homology search and DNA strand invasion. We report the purification of full-l...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Jie, Doty, Tammy, Gibson, Bryan, Heyer, Wolf-Dietrich
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2952495/
https://www.ncbi.nlm.nih.gov/pubmed/20729859
http://dx.doi.org/10.1038/nsmb.1904
_version_ 1782187783222198272
author Liu, Jie
Doty, Tammy
Gibson, Bryan
Heyer, Wolf-Dietrich
author_facet Liu, Jie
Doty, Tammy
Gibson, Bryan
Heyer, Wolf-Dietrich
author_sort Liu, Jie
collection PubMed
description BRCA2 is an important tumor suppressor and functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on ssDNA to perform homology search and DNA strand invasion. We report the purification of full-length human BRCA2, and show that it binds to ~6 RAD51 molecules and promotes RAD51 binding to RPA-covered ssDNA in a manner that is stimulated by DSS1.
format Text
id pubmed-2952495
institution National Center for Biotechnology Information
language English
publishDate 2010
record_format MEDLINE/PubMed
spelling pubmed-29524952011-04-01 Human BRCA2 protein promotes RAD51 filament formation on RPA-covered ssDNA Liu, Jie Doty, Tammy Gibson, Bryan Heyer, Wolf-Dietrich Nat Struct Mol Biol Article BRCA2 is an important tumor suppressor and functions in homologous recombination, a key genomic integrity pathway. BRCA2 interacts with RAD51, the central protein of recombination, which forms filaments on ssDNA to perform homology search and DNA strand invasion. We report the purification of full-length human BRCA2, and show that it binds to ~6 RAD51 molecules and promotes RAD51 binding to RPA-covered ssDNA in a manner that is stimulated by DSS1. 2010-08-22 2010-10 /pmc/articles/PMC2952495/ /pubmed/20729859 http://dx.doi.org/10.1038/nsmb.1904 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Liu, Jie
Doty, Tammy
Gibson, Bryan
Heyer, Wolf-Dietrich
Human BRCA2 protein promotes RAD51 filament formation on RPA-covered ssDNA
title Human BRCA2 protein promotes RAD51 filament formation on RPA-covered ssDNA
title_full Human BRCA2 protein promotes RAD51 filament formation on RPA-covered ssDNA
title_fullStr Human BRCA2 protein promotes RAD51 filament formation on RPA-covered ssDNA
title_full_unstemmed Human BRCA2 protein promotes RAD51 filament formation on RPA-covered ssDNA
title_short Human BRCA2 protein promotes RAD51 filament formation on RPA-covered ssDNA
title_sort human brca2 protein promotes rad51 filament formation on rpa-covered ssdna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2952495/
https://www.ncbi.nlm.nih.gov/pubmed/20729859
http://dx.doi.org/10.1038/nsmb.1904
work_keys_str_mv AT liujie humanbrca2proteinpromotesrad51filamentformationonrpacoveredssdna
AT dotytammy humanbrca2proteinpromotesrad51filamentformationonrpacoveredssdna
AT gibsonbryan humanbrca2proteinpromotesrad51filamentformationonrpacoveredssdna
AT heyerwolfdietrich humanbrca2proteinpromotesrad51filamentformationonrpacoveredssdna

Ejemplares similares