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Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules
Mitosis in eukaryotic cells employs spindle microtubules to drive accurate chromosome segregation at cell division. Cells lacking spindle microtubules arrest in mitosis due to a spindle checkpoint that delays mitotic progression until all chromosomes have achieved stable bipolar attachment to spindl...
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2953530/ https://www.ncbi.nlm.nih.gov/pubmed/20967237 http://dx.doi.org/10.1371/journal.pbio.1000512 |
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author | Castagnetti, Stefania Oliferenko, Snezhana Nurse, Paul |
author_facet | Castagnetti, Stefania Oliferenko, Snezhana Nurse, Paul |
author_sort | Castagnetti, Stefania |
collection | PubMed |
description | Mitosis in eukaryotic cells employs spindle microtubules to drive accurate chromosome segregation at cell division. Cells lacking spindle microtubules arrest in mitosis due to a spindle checkpoint that delays mitotic progression until all chromosomes have achieved stable bipolar attachment to spindle microtubules. In fission yeast, mitosis occurs within an intact nuclear membrane with the mitotic spindle elongating between the spindle pole bodies. We show here that in fission yeast interference with mitotic spindle formation delays mitosis only briefly and cells proceed to an unusual nuclear division process we term nuclear fission, during which cells perform some chromosome segregation and efficiently enter S-phase of the next cell cycle. Nuclear fission is blocked if spindle pole body maturation or sister chromatid separation cannot take place or if actin polymerization is inhibited. We suggest that this process exhibits vestiges of a primitive nuclear division process independent of spindle microtubules, possibly reflecting an evolutionary intermediate state between bacterial and Archeal chromosome segregation where the nucleoid divides without a spindle and a microtubule spindle-based eukaryotic mitosis. |
format | Text |
id | pubmed-2953530 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29535302010-10-21 Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules Castagnetti, Stefania Oliferenko, Snezhana Nurse, Paul PLoS Biol Research Article Mitosis in eukaryotic cells employs spindle microtubules to drive accurate chromosome segregation at cell division. Cells lacking spindle microtubules arrest in mitosis due to a spindle checkpoint that delays mitotic progression until all chromosomes have achieved stable bipolar attachment to spindle microtubules. In fission yeast, mitosis occurs within an intact nuclear membrane with the mitotic spindle elongating between the spindle pole bodies. We show here that in fission yeast interference with mitotic spindle formation delays mitosis only briefly and cells proceed to an unusual nuclear division process we term nuclear fission, during which cells perform some chromosome segregation and efficiently enter S-phase of the next cell cycle. Nuclear fission is blocked if spindle pole body maturation or sister chromatid separation cannot take place or if actin polymerization is inhibited. We suggest that this process exhibits vestiges of a primitive nuclear division process independent of spindle microtubules, possibly reflecting an evolutionary intermediate state between bacterial and Archeal chromosome segregation where the nucleoid divides without a spindle and a microtubule spindle-based eukaryotic mitosis. Public Library of Science 2010-10-12 /pmc/articles/PMC2953530/ /pubmed/20967237 http://dx.doi.org/10.1371/journal.pbio.1000512 Text en Castagnetti et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Castagnetti, Stefania Oliferenko, Snezhana Nurse, Paul Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules |
title | Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules |
title_full | Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules |
title_fullStr | Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules |
title_full_unstemmed | Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules |
title_short | Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules |
title_sort | fission yeast cells undergo nuclear division in the absence of spindle microtubules |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2953530/ https://www.ncbi.nlm.nih.gov/pubmed/20967237 http://dx.doi.org/10.1371/journal.pbio.1000512 |
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