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Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules

Mitosis in eukaryotic cells employs spindle microtubules to drive accurate chromosome segregation at cell division. Cells lacking spindle microtubules arrest in mitosis due to a spindle checkpoint that delays mitotic progression until all chromosomes have achieved stable bipolar attachment to spindl...

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Detalles Bibliográficos
Autores principales: Castagnetti, Stefania, Oliferenko, Snezhana, Nurse, Paul
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2953530/
https://www.ncbi.nlm.nih.gov/pubmed/20967237
http://dx.doi.org/10.1371/journal.pbio.1000512
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author Castagnetti, Stefania
Oliferenko, Snezhana
Nurse, Paul
author_facet Castagnetti, Stefania
Oliferenko, Snezhana
Nurse, Paul
author_sort Castagnetti, Stefania
collection PubMed
description Mitosis in eukaryotic cells employs spindle microtubules to drive accurate chromosome segregation at cell division. Cells lacking spindle microtubules arrest in mitosis due to a spindle checkpoint that delays mitotic progression until all chromosomes have achieved stable bipolar attachment to spindle microtubules. In fission yeast, mitosis occurs within an intact nuclear membrane with the mitotic spindle elongating between the spindle pole bodies. We show here that in fission yeast interference with mitotic spindle formation delays mitosis only briefly and cells proceed to an unusual nuclear division process we term nuclear fission, during which cells perform some chromosome segregation and efficiently enter S-phase of the next cell cycle. Nuclear fission is blocked if spindle pole body maturation or sister chromatid separation cannot take place or if actin polymerization is inhibited. We suggest that this process exhibits vestiges of a primitive nuclear division process independent of spindle microtubules, possibly reflecting an evolutionary intermediate state between bacterial and Archeal chromosome segregation where the nucleoid divides without a spindle and a microtubule spindle-based eukaryotic mitosis.
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spelling pubmed-29535302010-10-21 Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules Castagnetti, Stefania Oliferenko, Snezhana Nurse, Paul PLoS Biol Research Article Mitosis in eukaryotic cells employs spindle microtubules to drive accurate chromosome segregation at cell division. Cells lacking spindle microtubules arrest in mitosis due to a spindle checkpoint that delays mitotic progression until all chromosomes have achieved stable bipolar attachment to spindle microtubules. In fission yeast, mitosis occurs within an intact nuclear membrane with the mitotic spindle elongating between the spindle pole bodies. We show here that in fission yeast interference with mitotic spindle formation delays mitosis only briefly and cells proceed to an unusual nuclear division process we term nuclear fission, during which cells perform some chromosome segregation and efficiently enter S-phase of the next cell cycle. Nuclear fission is blocked if spindle pole body maturation or sister chromatid separation cannot take place or if actin polymerization is inhibited. We suggest that this process exhibits vestiges of a primitive nuclear division process independent of spindle microtubules, possibly reflecting an evolutionary intermediate state between bacterial and Archeal chromosome segregation where the nucleoid divides without a spindle and a microtubule spindle-based eukaryotic mitosis. Public Library of Science 2010-10-12 /pmc/articles/PMC2953530/ /pubmed/20967237 http://dx.doi.org/10.1371/journal.pbio.1000512 Text en Castagnetti et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Castagnetti, Stefania
Oliferenko, Snezhana
Nurse, Paul
Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules
title Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules
title_full Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules
title_fullStr Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules
title_full_unstemmed Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules
title_short Fission Yeast Cells Undergo Nuclear Division in the Absence of Spindle Microtubules
title_sort fission yeast cells undergo nuclear division in the absence of spindle microtubules
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2953530/
https://www.ncbi.nlm.nih.gov/pubmed/20967237
http://dx.doi.org/10.1371/journal.pbio.1000512
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