The structure of Jann_2411 (DUF1470) from Jannaschia sp. at 1.45 Å resolution reveals a new fold (the ABATE domain) and suggests its possible role as a transcription regulator

The crystal structure of Jann_2411 from Jannaschia sp. strain CCS1, a member of the Pfam PF07336 family classified as a domain of unknown function (DUF1470), was solved to a resolution of 1.45 Å by multiple-wavelength anomalous dispersion (MAD). This protein is the first structural representative of...

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Detalles Bibliográficos
Autores principales: Bakolitsa, Constantina, Bateman, Alex, Jin, Kevin K., McMullan, Daniel, Krishna, S. Sri, Miller, Mitchell D., Abdubek, Polat, Acosta, Claire, Astakhova, Tamara, Axelrod, Herbert L., Burra, Prasad, Carlton, Dennis, Chiu, Hsiu-Ju, Clayton, Thomas, Das, Debanu, Deller, Marc C., Duan, Lian, Elias, Ylva, Feuerhelm, Julie, Grant, Joanna C., Grzechnik, Anna, Grzechnik, Slawomir K., Han, Gye Won, Jaroszewski, Lukasz, Klock, Heath E., Knuth, Mark W., Kozbial, Piotr, Kumar, Abhinav, Marciano, David, Morse, Andrew T., Murphy, Kevin D., Nigoghossian, Edward, Okach, Linda, Oommachen, Silvya, Paulsen, Jessica, Reyes, Ron, Rife, Christopher L., Sefcovic, Natasha, Tien, Henry, Trame, Christine B., Trout, Christina V., van den Bedem, Henry, Weekes, Dana, White, Aprilfawn, Xu, Qingping, Hodgson, Keith O., Wooley, John, Elsliger, Marc-André, Deacon, Ashley M., Godzik, Adam, Lesley, Scott, Wilson, Ian A.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2009
Materias:
New Folds
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954205/
https://www.ncbi.nlm.nih.gov/pubmed/20944211
http://dx.doi.org/10.1107/S1744309109025196
Descripción
Sumario:The crystal structure of Jann_2411 from Jannaschia sp. strain CCS1, a member of the Pfam PF07336 family classified as a domain of unknown function (DUF1470), was solved to a resolution of 1.45 Å by multiple-wavelength anomalous dispersion (MAD). This protein is the first structural representative of the DUF1470 Pfam family. Structural analysis revealed a two-domain organization, with the N-terminal domain presenting a new fold called the ABATE domain that may bind an as yet unknown ligand. The C-terminal domain forms a treble-clef zinc finger that is likely to be involved in DNA binding. Analysis of the Jann_2411 protein and the broader ABATE-domain family suggests a role as stress-induced transcriptional regulators.

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