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The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism
The crystal structure of PA1994 from Pseudomonas aeruginosa, a member of the Pfam PF06475 family classified as a domain of unknown function (DUF1089), reveals a novel fold comprising a 15-stranded β-sheet wrapped around a single α-helix that assembles into a tight dimeric arrangement. The remote str...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
International Union of Crystallography
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954207/ https://www.ncbi.nlm.nih.gov/pubmed/20944213 http://dx.doi.org/10.1107/S1744309109022684 |
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| author | Bakolitsa, Constantina Kumar, Abhinav McMullan, Daniel Krishna, S. Sri Miller, Mitchell D. Carlton, Dennis Najmanovich, Rafael Abdubek, Polat Astakhova, Tamara Chiu, Hsiu-Ju Clayton, Thomas Deller, Marc C. Duan, Lian Elias, Ylva Feuerhelm, Julie Grant, Joanna C. Grzechnik, Slawomir K. Han, Gye Won Jaroszewski, Lukasz Jin, Kevin K. Klock, Heath E. Knuth, Mark W. Kozbial, Piotr Marciano, David Morse, Andrew T. Nigoghossian, Edward Okach, Linda Oommachen, Silvya Paulsen, Jessica Reyes, Ron Rife, Christopher L. Trout, Christina V. van den Bedem, Henry Weekes, Dana White, Aprilfawn Xu, Qingping Hodgson, Keith O. Wooley, John Elsliger, Marc-André Deacon, Ashley M. Godzik, Adam Lesley, Scott A. Wilson, Ian A. |
| author_facet | Bakolitsa, Constantina Kumar, Abhinav McMullan, Daniel Krishna, S. Sri Miller, Mitchell D. Carlton, Dennis Najmanovich, Rafael Abdubek, Polat Astakhova, Tamara Chiu, Hsiu-Ju Clayton, Thomas Deller, Marc C. Duan, Lian Elias, Ylva Feuerhelm, Julie Grant, Joanna C. Grzechnik, Slawomir K. Han, Gye Won Jaroszewski, Lukasz Jin, Kevin K. Klock, Heath E. Knuth, Mark W. Kozbial, Piotr Marciano, David Morse, Andrew T. Nigoghossian, Edward Okach, Linda Oommachen, Silvya Paulsen, Jessica Reyes, Ron Rife, Christopher L. Trout, Christina V. van den Bedem, Henry Weekes, Dana White, Aprilfawn Xu, Qingping Hodgson, Keith O. Wooley, John Elsliger, Marc-André Deacon, Ashley M. Godzik, Adam Lesley, Scott A. Wilson, Ian A. |
| author_sort | Bakolitsa, Constantina |
| collection | PubMed |
| description | The crystal structure of PA1994 from Pseudomonas aeruginosa, a member of the Pfam PF06475 family classified as a domain of unknown function (DUF1089), reveals a novel fold comprising a 15-stranded β-sheet wrapped around a single α-helix that assembles into a tight dimeric arrangement. The remote structural similarity to lipoprotein localization factors, in addition to the presence of an acidic pocket that is conserved in DUF1089 homologs, phospholipid-binding and sugar-binding proteins, indicate a role for PA1994 and the DUF1089 family in glycolipid metabolism. Genome-context analysis lends further support to the involvement of this family of proteins in glycolipid metabolism and indicates possible activation of DUF1089 homologs under conditions of bacterial cell-wall stress or host–pathogen interactions. |
| format | Text |
| id | pubmed-2954207 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2009 |
| publisher | International Union of Crystallography |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-29542072010-10-27 The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism Bakolitsa, Constantina Kumar, Abhinav McMullan, Daniel Krishna, S. Sri Miller, Mitchell D. Carlton, Dennis Najmanovich, Rafael Abdubek, Polat Astakhova, Tamara Chiu, Hsiu-Ju Clayton, Thomas Deller, Marc C. Duan, Lian Elias, Ylva Feuerhelm, Julie Grant, Joanna C. Grzechnik, Slawomir K. Han, Gye Won Jaroszewski, Lukasz Jin, Kevin K. Klock, Heath E. Knuth, Mark W. Kozbial, Piotr Marciano, David Morse, Andrew T. Nigoghossian, Edward Okach, Linda Oommachen, Silvya Paulsen, Jessica Reyes, Ron Rife, Christopher L. Trout, Christina V. van den Bedem, Henry Weekes, Dana White, Aprilfawn Xu, Qingping Hodgson, Keith O. Wooley, John Elsliger, Marc-André Deacon, Ashley M. Godzik, Adam Lesley, Scott A. Wilson, Ian A. Acta Crystallogr Sect F Struct Biol Cryst Commun New Folds The crystal structure of PA1994 from Pseudomonas aeruginosa, a member of the Pfam PF06475 family classified as a domain of unknown function (DUF1089), reveals a novel fold comprising a 15-stranded β-sheet wrapped around a single α-helix that assembles into a tight dimeric arrangement. The remote structural similarity to lipoprotein localization factors, in addition to the presence of an acidic pocket that is conserved in DUF1089 homologs, phospholipid-binding and sugar-binding proteins, indicate a role for PA1994 and the DUF1089 family in glycolipid metabolism. Genome-context analysis lends further support to the involvement of this family of proteins in glycolipid metabolism and indicates possible activation of DUF1089 homologs under conditions of bacterial cell-wall stress or host–pathogen interactions. International Union of Crystallography 2009-10-27 /pmc/articles/PMC2954207/ /pubmed/20944213 http://dx.doi.org/10.1107/S1744309109022684 Text en © Bakolitsa et al. 2010 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
| spellingShingle | New Folds Bakolitsa, Constantina Kumar, Abhinav McMullan, Daniel Krishna, S. Sri Miller, Mitchell D. Carlton, Dennis Najmanovich, Rafael Abdubek, Polat Astakhova, Tamara Chiu, Hsiu-Ju Clayton, Thomas Deller, Marc C. Duan, Lian Elias, Ylva Feuerhelm, Julie Grant, Joanna C. Grzechnik, Slawomir K. Han, Gye Won Jaroszewski, Lukasz Jin, Kevin K. Klock, Heath E. Knuth, Mark W. Kozbial, Piotr Marciano, David Morse, Andrew T. Nigoghossian, Edward Okach, Linda Oommachen, Silvya Paulsen, Jessica Reyes, Ron Rife, Christopher L. Trout, Christina V. van den Bedem, Henry Weekes, Dana White, Aprilfawn Xu, Qingping Hodgson, Keith O. Wooley, John Elsliger, Marc-André Deacon, Ashley M. Godzik, Adam Lesley, Scott A. Wilson, Ian A. The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism |
| title | The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism |
| title_full | The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism |
| title_fullStr | The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism |
| title_full_unstemmed | The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism |
| title_short | The structure of the first representative of Pfam family PF06475 reveals a new fold with possible involvement in glycolipid metabolism |
| title_sort | structure of the first representative of pfam family pf06475 reveals a new fold with possible involvement in glycolipid metabolism |
| topic | New Folds |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954207/ https://www.ncbi.nlm.nih.gov/pubmed/20944213 http://dx.doi.org/10.1107/S1744309109022684 |
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