Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster

A novel aminoacyl-tRNA synthetase that contains an iron–sulfur cluster in the tRNA anticodon-binding region and efficiently charges tRNA with tryptophan has been found in Thermotoga maritima. The crystal structure of TmTrpRS (tryptophanyl-tRNA synthetase; TrpRS; EC 6.1.1.2) reveals an iron–sulfur [4...

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Autores principales: Han, Gye Won, Yang, Xiang-Lei, McMullan, Daniel, Chong, Yeeting E., Krishna, S. Sri, Rife, Christopher L., Weekes, Dana, Brittain, Scott M., Abdubek, Polat, Ambing, Eileen, Astakhova, Tamara, Axelrod, Herbert L., Carlton, Dennis, Caruthers, Jonathan, Chiu, Hsiu-Ju, Clayton, Thomas, Duan, Lian, Feuerhelm, Julie, Grant, Joanna C., Grzechnik, Slawomir K., Jaroszewski, Lukasz, Jin, Kevin K., Klock, Heath E., Knuth, Mark W., Kumar, Abhinav, Marciano, David, Miller, Mitchell D., Morse, Andrew T., Nigoghossian, Edward, Okach, Linda, Paulsen, Jessica, Reyes, Ron, van den Bedem, Henry, White, Aprilfawn, Wolf, Guenter, Xu, Qingping, Hodgson, Keith O., Wooley, John, Deacon, Ashley M., Godzik, Adam, Lesley, Scott A., Elsliger, Marc-André, Schimmel, Paul, Wilson, Ian A.
Formato: Texto
Lenguaje:English
Publicado: International Union of Crystallography 2010
Materias:
Ligands That Aid in Function Characterization
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954223/
https://www.ncbi.nlm.nih.gov/pubmed/20944229
http://dx.doi.org/10.1107/S1744309110037619
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author Han, Gye Won
Yang, Xiang-Lei
McMullan, Daniel
Chong, Yeeting E.
Krishna, S. Sri
Rife, Christopher L.
Weekes, Dana
Brittain, Scott M.
Abdubek, Polat
Ambing, Eileen
Astakhova, Tamara
Axelrod, Herbert L.
Carlton, Dennis
Caruthers, Jonathan
Chiu, Hsiu-Ju
Clayton, Thomas
Duan, Lian
Feuerhelm, Julie
Grant, Joanna C.
Grzechnik, Slawomir K.
Jaroszewski, Lukasz
Jin, Kevin K.
Klock, Heath E.
Knuth, Mark W.
Kumar, Abhinav
Marciano, David
Miller, Mitchell D.
Morse, Andrew T.
Nigoghossian, Edward
Okach, Linda
Paulsen, Jessica
Reyes, Ron
van den Bedem, Henry
White, Aprilfawn
Wolf, Guenter
Xu, Qingping
Hodgson, Keith O.
Wooley, John
Deacon, Ashley M.
Godzik, Adam
Lesley, Scott A.
Elsliger, Marc-André
Schimmel, Paul
Wilson, Ian A.
author_facet Han, Gye Won
Yang, Xiang-Lei
McMullan, Daniel
Chong, Yeeting E.
Krishna, S. Sri
Rife, Christopher L.
Weekes, Dana
Brittain, Scott M.
Abdubek, Polat
Ambing, Eileen
Astakhova, Tamara
Axelrod, Herbert L.
Carlton, Dennis
Caruthers, Jonathan
Chiu, Hsiu-Ju
Clayton, Thomas
Duan, Lian
Feuerhelm, Julie
Grant, Joanna C.
Grzechnik, Slawomir K.
Jaroszewski, Lukasz
Jin, Kevin K.
Klock, Heath E.
Knuth, Mark W.
Kumar, Abhinav
Marciano, David
Miller, Mitchell D.
Morse, Andrew T.
Nigoghossian, Edward
Okach, Linda
Paulsen, Jessica
Reyes, Ron
van den Bedem, Henry
White, Aprilfawn
Wolf, Guenter
Xu, Qingping
Hodgson, Keith O.
Wooley, John
Deacon, Ashley M.
Godzik, Adam
Lesley, Scott A.
Elsliger, Marc-André
Schimmel, Paul
Wilson, Ian A.
author_sort Han, Gye Won
collection PubMed
description A novel aminoacyl-tRNA synthetase that contains an iron–sulfur cluster in the tRNA anticodon-binding region and efficiently charges tRNA with tryptophan has been found in Thermotoga maritima. The crystal structure of TmTrpRS (tryptophanyl-tRNA synthetase; TrpRS; EC 6.1.1.2) reveals an iron–sulfur [4Fe–­4S] cluster bound to the tRNA anticodon-binding (TAB) domain and an l-­tryptophan ligand in the active site. None of the other T. maritima aminoacyl-tRNA synthetases (AARSs) contain this [4Fe–4S] cluster-binding motif (C-x (22)-C-x (6)-C-x (2)-C). It is speculated that the iron–sulfur cluster contributes to the stability of TmTrpRS and could play a role in the recognition of the anticodon.
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spelling pubmed-29542232010-10-27 Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster Han, Gye Won Yang, Xiang-Lei McMullan, Daniel Chong, Yeeting E. Krishna, S. Sri Rife, Christopher L. Weekes, Dana Brittain, Scott M. Abdubek, Polat Ambing, Eileen Astakhova, Tamara Axelrod, Herbert L. Carlton, Dennis Caruthers, Jonathan Chiu, Hsiu-Ju Clayton, Thomas Duan, Lian Feuerhelm, Julie Grant, Joanna C. Grzechnik, Slawomir K. Jaroszewski, Lukasz Jin, Kevin K. Klock, Heath E. Knuth, Mark W. Kumar, Abhinav Marciano, David Miller, Mitchell D. Morse, Andrew T. Nigoghossian, Edward Okach, Linda Paulsen, Jessica Reyes, Ron van den Bedem, Henry White, Aprilfawn Wolf, Guenter Xu, Qingping Hodgson, Keith O. Wooley, John Deacon, Ashley M. Godzik, Adam Lesley, Scott A. Elsliger, Marc-André Schimmel, Paul Wilson, Ian A. Acta Crystallogr Sect F Struct Biol Cryst Commun Ligands That Aid in Function Characterization A novel aminoacyl-tRNA synthetase that contains an iron–sulfur cluster in the tRNA anticodon-binding region and efficiently charges tRNA with tryptophan has been found in Thermotoga maritima. The crystal structure of TmTrpRS (tryptophanyl-tRNA synthetase; TrpRS; EC 6.1.1.2) reveals an iron–sulfur [4Fe–­4S] cluster bound to the tRNA anticodon-binding (TAB) domain and an l-­tryptophan ligand in the active site. None of the other T. maritima aminoacyl-tRNA synthetases (AARSs) contain this [4Fe–4S] cluster-binding motif (C-x (22)-C-x (6)-C-x (2)-C). It is speculated that the iron–sulfur cluster contributes to the stability of TmTrpRS and could play a role in the recognition of the anticodon. International Union of Crystallography 2010-09-23 /pmc/articles/PMC2954223/ /pubmed/20944229 http://dx.doi.org/10.1107/S1744309110037619 Text en © Han et al. 2010 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Ligands That Aid in Function Characterization
Han, Gye Won
Yang, Xiang-Lei
McMullan, Daniel
Chong, Yeeting E.
Krishna, S. Sri
Rife, Christopher L.
Weekes, Dana
Brittain, Scott M.
Abdubek, Polat
Ambing, Eileen
Astakhova, Tamara
Axelrod, Herbert L.
Carlton, Dennis
Caruthers, Jonathan
Chiu, Hsiu-Ju
Clayton, Thomas
Duan, Lian
Feuerhelm, Julie
Grant, Joanna C.
Grzechnik, Slawomir K.
Jaroszewski, Lukasz
Jin, Kevin K.
Klock, Heath E.
Knuth, Mark W.
Kumar, Abhinav
Marciano, David
Miller, Mitchell D.
Morse, Andrew T.
Nigoghossian, Edward
Okach, Linda
Paulsen, Jessica
Reyes, Ron
van den Bedem, Henry
White, Aprilfawn
Wolf, Guenter
Xu, Qingping
Hodgson, Keith O.
Wooley, John
Deacon, Ashley M.
Godzik, Adam
Lesley, Scott A.
Elsliger, Marc-André
Schimmel, Paul
Wilson, Ian A.
Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster
title Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster
title_full Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster
title_fullStr Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster
title_full_unstemmed Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster
title_short Structure of a tryptophanyl-tRNA synthetase containing an iron–sulfur cluster
title_sort structure of a tryptophanyl-trna synthetase containing an iron–sulfur cluster
topic Ligands That Aid in Function Characterization
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954223/
https://www.ncbi.nlm.nih.gov/pubmed/20944229
http://dx.doi.org/10.1107/S1744309110037619
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