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Development of a serum-free medium for in vitro expansion of human cytotoxic T lymphocytes using a statistical design
BACKGROUND: Serum-containing medium (SCM), which has a number of poorly defined components with varying concentrations, hampers standardization of lymphocyte cultures. In order to develop a serum-free medium (SFM) for the expansion of human lymphocytes from peripheral blood mononuclear cells (PBMCs)...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954940/ https://www.ncbi.nlm.nih.gov/pubmed/20854694 http://dx.doi.org/10.1186/1472-6750-10-70 |