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Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1
BACKGROUND: Bacteriophages (phages) are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens. RESULTS: LSB-1, a reference coliphage strain, was classified as a member of the Podoviridae family with a cystic form (50 ± 5 nm...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2955717/ https://www.ncbi.nlm.nih.gov/pubmed/20875143 http://dx.doi.org/10.1186/1743-422X-7-255 |
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author | Chai, Yaming Xiong, Hongyan Ma, Xiangyu Cheng, Liqing Huang, Guorong Zhang, Zhonglin Rao Lin |
author_facet | Chai, Yaming Xiong, Hongyan Ma, Xiangyu Cheng, Liqing Huang, Guorong Zhang, Zhonglin Rao Lin |
author_sort | Chai, Yaming |
collection | PubMed |
description | BACKGROUND: Bacteriophages (phages) are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens. RESULTS: LSB-1, a reference coliphage strain, was classified as a member of the Podoviridae family with a cystic form (50 ± 5 nm diameter) and short tail (60 ± 5 nm long). The double stranded DNA was about 30 kilobase pairs in length. We identified its host range and determined the gp17 sequences and protein structure using shotgun analysis and bioinformatics technology. CONCLUSIONS: Coliphage LSB-1 possesses a tailspike protein with endosialidase activity which is probably responsible for its specific enteroinvasive E.coli host range within the laboratory. |
format | Text |
id | pubmed-2955717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29557172010-10-16 Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1 Chai, Yaming Xiong, Hongyan Ma, Xiangyu Cheng, Liqing Huang, Guorong Zhang, Zhonglin Rao Lin Virol J Research BACKGROUND: Bacteriophages (phages) are widespread in the environment and play a crucial role in the evolution of their bacterial hosts and the emergence of new pathogens. RESULTS: LSB-1, a reference coliphage strain, was classified as a member of the Podoviridae family with a cystic form (50 ± 5 nm diameter) and short tail (60 ± 5 nm long). The double stranded DNA was about 30 kilobase pairs in length. We identified its host range and determined the gp17 sequences and protein structure using shotgun analysis and bioinformatics technology. CONCLUSIONS: Coliphage LSB-1 possesses a tailspike protein with endosialidase activity which is probably responsible for its specific enteroinvasive E.coli host range within the laboratory. BioMed Central 2010-09-28 /pmc/articles/PMC2955717/ /pubmed/20875143 http://dx.doi.org/10.1186/1743-422X-7-255 Text en Copyright ©2010 Chai et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Chai, Yaming Xiong, Hongyan Ma, Xiangyu Cheng, Liqing Huang, Guorong Zhang, Zhonglin Rao Lin Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1 |
title | Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1 |
title_full | Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1 |
title_fullStr | Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1 |
title_full_unstemmed | Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1 |
title_short | Molecular characterization, structural analysis and determination of host range of a novel bacteriophage LSB-1 |
title_sort | molecular characterization, structural analysis and determination of host range of a novel bacteriophage lsb-1 |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2955717/ https://www.ncbi.nlm.nih.gov/pubmed/20875143 http://dx.doi.org/10.1186/1743-422X-7-255 |
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