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The Mechanism of (R,R) ZX-5 on Increasing NO Release
(R,R) ZX-5 has been proven to have positive effects on choroidal blood flow without affecting the sclera and ciliary bodies in New Zealand white rabbits. This study was designed to investigate the mechanisms of (R,R) ZX-5 on improving the choroidal blood flow and promoting NO production. HUVECs (hum...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956097/ https://www.ncbi.nlm.nih.gov/pubmed/20957097 http://dx.doi.org/10.3390/ijms11093323 |
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author | Xu, Han-Mei Wei, Jin Pan, Li Lin, Hongying Wang, Weiqiang Zhang, Yihua Shen, Zilong |
author_facet | Xu, Han-Mei Wei, Jin Pan, Li Lin, Hongying Wang, Weiqiang Zhang, Yihua Shen, Zilong |
author_sort | Xu, Han-Mei |
collection | PubMed |
description | (R,R) ZX-5 has been proven to have positive effects on choroidal blood flow without affecting the sclera and ciliary bodies in New Zealand white rabbits. This study was designed to investigate the mechanisms of (R,R) ZX-5 on improving the choroidal blood flow and promoting NO production. HUVECs (human umbilical vein endothelial cells) were used to determine the production of eNOS, p-eNOS, AKT and Erk1/2 by Western blot analysis. iNOS and eNOS mRNA levels were investigated by RT-PCR and the effect of (R,R) ZX-5 on NO production were determined by eNOS activity assay. We found (R,R) ZX-5 upregulated protein expression of eNOS and iNOS, increased NO production, and reduced ERK and Akt protein level. Therefore, (R,R) ZX-5 may promote the choroidal blood flow in New Zealand white rabbits without affecting the blood flow in the iris or ciliary bodies via increasing NO production. These results suggest that (R,R) ZX-5 may function to cure and prevent Age-related macular degeneration (AMD). |
format | Text |
id | pubmed-2956097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-29560972010-10-18 The Mechanism of (R,R) ZX-5 on Increasing NO Release Xu, Han-Mei Wei, Jin Pan, Li Lin, Hongying Wang, Weiqiang Zhang, Yihua Shen, Zilong Int J Mol Sci Article (R,R) ZX-5 has been proven to have positive effects on choroidal blood flow without affecting the sclera and ciliary bodies in New Zealand white rabbits. This study was designed to investigate the mechanisms of (R,R) ZX-5 on improving the choroidal blood flow and promoting NO production. HUVECs (human umbilical vein endothelial cells) were used to determine the production of eNOS, p-eNOS, AKT and Erk1/2 by Western blot analysis. iNOS and eNOS mRNA levels were investigated by RT-PCR and the effect of (R,R) ZX-5 on NO production were determined by eNOS activity assay. We found (R,R) ZX-5 upregulated protein expression of eNOS and iNOS, increased NO production, and reduced ERK and Akt protein level. Therefore, (R,R) ZX-5 may promote the choroidal blood flow in New Zealand white rabbits without affecting the blood flow in the iris or ciliary bodies via increasing NO production. These results suggest that (R,R) ZX-5 may function to cure and prevent Age-related macular degeneration (AMD). Molecular Diversity Preservation International (MDPI) 2010-09-15 /pmc/articles/PMC2956097/ /pubmed/20957097 http://dx.doi.org/10.3390/ijms11093323 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Xu, Han-Mei Wei, Jin Pan, Li Lin, Hongying Wang, Weiqiang Zhang, Yihua Shen, Zilong The Mechanism of (R,R) ZX-5 on Increasing NO Release |
title | The Mechanism of (R,R) ZX-5 on Increasing NO Release |
title_full | The Mechanism of (R,R) ZX-5 on Increasing NO Release |
title_fullStr | The Mechanism of (R,R) ZX-5 on Increasing NO Release |
title_full_unstemmed | The Mechanism of (R,R) ZX-5 on Increasing NO Release |
title_short | The Mechanism of (R,R) ZX-5 on Increasing NO Release |
title_sort | mechanism of (r,r) zx-5 on increasing no release |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956097/ https://www.ncbi.nlm.nih.gov/pubmed/20957097 http://dx.doi.org/10.3390/ijms11093323 |
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