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Rapid Detection of Reassortment of Pandemic H1N1/2009 Influenza Virus

BACKGROUND: Influenza viruses can generate novel reassortants in coinfected cells. The global circulation and occasional introductions of pandemic H1N1/2009 virus in humans and in pigs, respectively, may allow this virus to reassort with other influenza viruses. These possible reassortment events mi...

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Autores principales: Poon, Leo L M, Mak, Polly W Y, Li, Olive T W, Chan, Kwok Hung, Cheung, Chung Lam, Ma, Edward S, Yen, Hui-Ling, Vijaykrishna, Dhanasekaran, Guan, Yi, Peiris, J S Malik
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956410/
https://www.ncbi.nlm.nih.gov/pubmed/20567024
http://dx.doi.org/10.1373/clinchem.2010.149179
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author Poon, Leo L M
Mak, Polly W Y
Li, Olive T W
Chan, Kwok Hung
Cheung, Chung Lam
Ma, Edward S
Yen, Hui-Ling
Vijaykrishna, Dhanasekaran
Guan, Yi
Peiris, J S Malik
author_facet Poon, Leo L M
Mak, Polly W Y
Li, Olive T W
Chan, Kwok Hung
Cheung, Chung Lam
Ma, Edward S
Yen, Hui-Ling
Vijaykrishna, Dhanasekaran
Guan, Yi
Peiris, J S Malik
author_sort Poon, Leo L M
collection PubMed
description BACKGROUND: Influenza viruses can generate novel reassortants in coinfected cells. The global circulation and occasional introductions of pandemic H1N1/2009 virus in humans and in pigs, respectively, may allow this virus to reassort with other influenza viruses. These possible reassortment events might alter virulence and/or transmissibility of the new reassortants. Investigations to detect such possible reassortants should be included as a part of pandemic influenza surveillance plans. METHODS: We established a real-time reverse-transcription (RT)-PCR–based strategy for the detection of reassortment of pandemic H1N1/2009 virus. Singleplex SYBR green–based RT-PCR assays specific for each gene segment of pandemic H1N1/2009 were developed. These assays were evaluated with influenza viruses of various genetic backgrounds. RESULTS: All human pandemic H1N1 (n = 27) and all seasonal human (n = 58) isolates were positive and negative, respectively, for all 8 segments. Of 48 swine influenza viruses isolated from our ongoing surveillance program of influenza viruses in swine, 10 were positive in all reactions. All 8 viral segments of these 10 samples were confirmed to be of pandemic H1N1 origin, indicating that these were caused by zoonotic transmissions from human to pigs. The 38 swine viruses that were nonpandemic H1N1/2009 had 1–6 gene segments positive in the tests. Further characterization of these nonpandemic H1N1/2009 swine viruses indicated that these PCR-positive genes were the precursor genes of the pandemic H1N1/2009 virus. CONCLUSIONS: Our results demonstrated that these assays can detect reintroductions of pandemic H1N1/2009 virus in pigs. These assays might be useful screening tools for identifying viral reassortants derived from pandemic H1N1/2009 or its precursors.
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spelling pubmed-29564102011-08-01 Rapid Detection of Reassortment of Pandemic H1N1/2009 Influenza Virus Poon, Leo L M Mak, Polly W Y Li, Olive T W Chan, Kwok Hung Cheung, Chung Lam Ma, Edward S Yen, Hui-Ling Vijaykrishna, Dhanasekaran Guan, Yi Peiris, J S Malik Clin Chem Brief Communications BACKGROUND: Influenza viruses can generate novel reassortants in coinfected cells. The global circulation and occasional introductions of pandemic H1N1/2009 virus in humans and in pigs, respectively, may allow this virus to reassort with other influenza viruses. These possible reassortment events might alter virulence and/or transmissibility of the new reassortants. Investigations to detect such possible reassortants should be included as a part of pandemic influenza surveillance plans. METHODS: We established a real-time reverse-transcription (RT)-PCR–based strategy for the detection of reassortment of pandemic H1N1/2009 virus. Singleplex SYBR green–based RT-PCR assays specific for each gene segment of pandemic H1N1/2009 were developed. These assays were evaluated with influenza viruses of various genetic backgrounds. RESULTS: All human pandemic H1N1 (n = 27) and all seasonal human (n = 58) isolates were positive and negative, respectively, for all 8 segments. Of 48 swine influenza viruses isolated from our ongoing surveillance program of influenza viruses in swine, 10 were positive in all reactions. All 8 viral segments of these 10 samples were confirmed to be of pandemic H1N1 origin, indicating that these were caused by zoonotic transmissions from human to pigs. The 38 swine viruses that were nonpandemic H1N1/2009 had 1–6 gene segments positive in the tests. Further characterization of these nonpandemic H1N1/2009 swine viruses indicated that these PCR-positive genes were the precursor genes of the pandemic H1N1/2009 virus. CONCLUSIONS: Our results demonstrated that these assays can detect reintroductions of pandemic H1N1/2009 virus in pigs. These assays might be useful screening tools for identifying viral reassortants derived from pandemic H1N1/2009 or its precursors. Oxford University Press 2010-08 2010-08-01 /pmc/articles/PMC2956410/ /pubmed/20567024 http://dx.doi.org/10.1373/clinchem.2010.149179 Text en © 2010 The American Association for Clinical Chemistry https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.
spellingShingle Brief Communications
Poon, Leo L M
Mak, Polly W Y
Li, Olive T W
Chan, Kwok Hung
Cheung, Chung Lam
Ma, Edward S
Yen, Hui-Ling
Vijaykrishna, Dhanasekaran
Guan, Yi
Peiris, J S Malik
Rapid Detection of Reassortment of Pandemic H1N1/2009 Influenza Virus
title Rapid Detection of Reassortment of Pandemic H1N1/2009 Influenza Virus
title_full Rapid Detection of Reassortment of Pandemic H1N1/2009 Influenza Virus
title_fullStr Rapid Detection of Reassortment of Pandemic H1N1/2009 Influenza Virus
title_full_unstemmed Rapid Detection of Reassortment of Pandemic H1N1/2009 Influenza Virus
title_short Rapid Detection of Reassortment of Pandemic H1N1/2009 Influenza Virus
title_sort rapid detection of reassortment of pandemic h1n1/2009 influenza virus
topic Brief Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956410/
https://www.ncbi.nlm.nih.gov/pubmed/20567024
http://dx.doi.org/10.1373/clinchem.2010.149179
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