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Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia

BACKGROUND: It is widely recognized that interspecific hybridization may induce "genome shock", and lead to genetic and epigenetic instabilities in the resultant hybrids and/or backcrossed introgressants. A prominent component involved in the genome shock is reactivation of cryptic transpo...

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Autores principales: Wang, Ningning, Wang, Hongyan, Wang, Hui, Zhang, Di, Wu, Ying, Ou, Xiufang, Liu, Shuang, Dong, Zhenying, Liu, Bao
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956540/
https://www.ncbi.nlm.nih.gov/pubmed/20796287
http://dx.doi.org/10.1186/1471-2229-10-190
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author Wang, Ningning
Wang, Hongyan
Wang, Hui
Zhang, Di
Wu, Ying
Ou, Xiufang
Liu, Shuang
Dong, Zhenying
Liu, Bao
author_facet Wang, Ningning
Wang, Hongyan
Wang, Hui
Zhang, Di
Wu, Ying
Ou, Xiufang
Liu, Shuang
Dong, Zhenying
Liu, Bao
author_sort Wang, Ningning
collection PubMed
description BACKGROUND: It is widely recognized that interspecific hybridization may induce "genome shock", and lead to genetic and epigenetic instabilities in the resultant hybrids and/or backcrossed introgressants. A prominent component involved in the genome shock is reactivation of cryptic transposable elements (TEs) in the hybrid genome, which is often associated with alteration in the elements' epigenetic modifications like cytosine DNA methylation. We have previously reported that introgressants derived from hybridization between Oryza sativa (rice) and Zizania latifolia manifested substantial methylation re-patterning and rampant mobilization of two TEs, a copia retrotransposon Tos17 and a MITE mPing. It was not known however whether other types of TEs had also been transpositionally reactivated in these introgressants, their relevance to alteration in cytosine methylation, and their impact on expression of adjacent cellular genes. RESULTS: We document in this study that the Dart TE family was transpositionally reactivated followed by stabilization in all three studied introgressants (RZ1, RZ2 and RZ35) derived from introgressive hybridization between rice (cv. Matsumae) and Z. latifolia, while the TEs remained quiescent in the recipient rice genome. Transposon-display (TD) and sequencing verified the element's mobility and mapped the excisions and re-insertions to the rice chromosomes. Methylation-sensitive Southern blotting showed that the Dart TEs were heavily methylated along their entire length, and moderate alteration in cytosine methylation patterns occurred in the introgressants relative to their rice parental line. Real-time qRT-PCR quantification on the relative transcript abundance of six single-copy genes flanking the newly excised or inserted Dart-related TE copies indicated that whereas marked difference in the expression of all four genes in both tissues (leaf and root) were detected between the introgressants and their rice parental line under both normal and various stress conditions, the difference showed little association with the presence or absence of the newly mobilized Dart-related TEs. CONCLUSION: Introgressive hybridization has induced transpositional reactivation of the otherwise immobile Dart-related TEs in the parental rice line (cv. Matsumae), which was accompanied with a moderate alteration in the element's cytosine methylation. Significant difference in expression of the Dart-adjacent genes occurred between the introgressants and their rice parental line under both normal and various abiotic stress conditions, but the alteration in gene expression was not coupled with the TEs.
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spelling pubmed-29565402010-10-19 Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia Wang, Ningning Wang, Hongyan Wang, Hui Zhang, Di Wu, Ying Ou, Xiufang Liu, Shuang Dong, Zhenying Liu, Bao BMC Plant Biol Research Article BACKGROUND: It is widely recognized that interspecific hybridization may induce "genome shock", and lead to genetic and epigenetic instabilities in the resultant hybrids and/or backcrossed introgressants. A prominent component involved in the genome shock is reactivation of cryptic transposable elements (TEs) in the hybrid genome, which is often associated with alteration in the elements' epigenetic modifications like cytosine DNA methylation. We have previously reported that introgressants derived from hybridization between Oryza sativa (rice) and Zizania latifolia manifested substantial methylation re-patterning and rampant mobilization of two TEs, a copia retrotransposon Tos17 and a MITE mPing. It was not known however whether other types of TEs had also been transpositionally reactivated in these introgressants, their relevance to alteration in cytosine methylation, and their impact on expression of adjacent cellular genes. RESULTS: We document in this study that the Dart TE family was transpositionally reactivated followed by stabilization in all three studied introgressants (RZ1, RZ2 and RZ35) derived from introgressive hybridization between rice (cv. Matsumae) and Z. latifolia, while the TEs remained quiescent in the recipient rice genome. Transposon-display (TD) and sequencing verified the element's mobility and mapped the excisions and re-insertions to the rice chromosomes. Methylation-sensitive Southern blotting showed that the Dart TEs were heavily methylated along their entire length, and moderate alteration in cytosine methylation patterns occurred in the introgressants relative to their rice parental line. Real-time qRT-PCR quantification on the relative transcript abundance of six single-copy genes flanking the newly excised or inserted Dart-related TE copies indicated that whereas marked difference in the expression of all four genes in both tissues (leaf and root) were detected between the introgressants and their rice parental line under both normal and various stress conditions, the difference showed little association with the presence or absence of the newly mobilized Dart-related TEs. CONCLUSION: Introgressive hybridization has induced transpositional reactivation of the otherwise immobile Dart-related TEs in the parental rice line (cv. Matsumae), which was accompanied with a moderate alteration in the element's cytosine methylation. Significant difference in expression of the Dart-adjacent genes occurred between the introgressants and their rice parental line under both normal and various abiotic stress conditions, but the alteration in gene expression was not coupled with the TEs. BioMed Central 2010-08-26 /pmc/articles/PMC2956540/ /pubmed/20796287 http://dx.doi.org/10.1186/1471-2229-10-190 Text en Copyright ©2010 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Ningning
Wang, Hongyan
Wang, Hui
Zhang, Di
Wu, Ying
Ou, Xiufang
Liu, Shuang
Dong, Zhenying
Liu, Bao
Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia
title Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia
title_full Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia
title_fullStr Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia
title_full_unstemmed Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia
title_short Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia
title_sort transpositional reactivation of the dart transposon family in rice lines derived from introgressive hybridization with zizania latifolia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956540/
https://www.ncbi.nlm.nih.gov/pubmed/20796287
http://dx.doi.org/10.1186/1471-2229-10-190
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