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Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice

BACKGROUND: Berberine, an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-inflammatory and neuroprotective effects. However, there are no reports about the effects and mechanisms of berberine in experimental autoimmune encephalomyelitis...

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Autores principales: Ma, Xiaomeng, Jiang, Ying, Wu, Aimin, Chen, Xiaohong, Pi, Rongbiao, Liu, Mei, Liu, Yingying
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957444/
https://www.ncbi.nlm.nih.gov/pubmed/20976070
http://dx.doi.org/10.1371/journal.pone.0013489
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author Ma, Xiaomeng
Jiang, Ying
Wu, Aimin
Chen, Xiaohong
Pi, Rongbiao
Liu, Mei
Liu, Yingying
author_facet Ma, Xiaomeng
Jiang, Ying
Wu, Aimin
Chen, Xiaohong
Pi, Rongbiao
Liu, Mei
Liu, Yingying
author_sort Ma, Xiaomeng
collection PubMed
description BACKGROUND: Berberine, an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-inflammatory and neuroprotective effects. However, there are no reports about the effects and mechanisms of berberine in experimental autoimmune encephalomyelitis (EAE), an established model of multiple sclerosis (MS). METHODOLOGY/PRINCIPAL FINDINGS: Female C57 BL/6 mice immunized with myelin oligodendrocyte glycoprotein 35–55 amino acid peptide were treated with berberine at the day of disease onset and medication was administered daily until mice were sacrificed. Blood–brain barrier (BBB) permeability and the alteration of matrix metalloproteinase-2 (MMP-2, 72 kDa) and matrix metalloproteinase-9 (MMP-9, 92 kDa) in the brain and cerebrospinal fluid (CSF) of EAE mice were detected by quantitative measurement for Evan's blue (EB) content, Western blot and gelatin zymography respectively. The results showed that berberine attenuated clinical and pathological parameters of EAE, reduced the permeability of BBB, inhibited the activity and expression of MMP-9 but not MMP-2 in the CSF and brain of EAE mice. CONCLUSIONS/SIGNIFICANCE: These findings suggest that berberine is effective to attenuate the clinical severity of EAE in C57 BL/6 mice by reducing the permeability of BBB, decreasing the expression and activity of MMP-9, and decreasing the inflammatory infiltration. We think that berberine might be a potential therapeutic agent for MS.
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spelling pubmed-29574442010-10-25 Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice Ma, Xiaomeng Jiang, Ying Wu, Aimin Chen, Xiaohong Pi, Rongbiao Liu, Mei Liu, Yingying PLoS One Research Article BACKGROUND: Berberine, an isoquinoline derivative alkaloid, has a wide range of pharmacological properties and is considered to have anti-inflammatory and neuroprotective effects. However, there are no reports about the effects and mechanisms of berberine in experimental autoimmune encephalomyelitis (EAE), an established model of multiple sclerosis (MS). METHODOLOGY/PRINCIPAL FINDINGS: Female C57 BL/6 mice immunized with myelin oligodendrocyte glycoprotein 35–55 amino acid peptide were treated with berberine at the day of disease onset and medication was administered daily until mice were sacrificed. Blood–brain barrier (BBB) permeability and the alteration of matrix metalloproteinase-2 (MMP-2, 72 kDa) and matrix metalloproteinase-9 (MMP-9, 92 kDa) in the brain and cerebrospinal fluid (CSF) of EAE mice were detected by quantitative measurement for Evan's blue (EB) content, Western blot and gelatin zymography respectively. The results showed that berberine attenuated clinical and pathological parameters of EAE, reduced the permeability of BBB, inhibited the activity and expression of MMP-9 but not MMP-2 in the CSF and brain of EAE mice. CONCLUSIONS/SIGNIFICANCE: These findings suggest that berberine is effective to attenuate the clinical severity of EAE in C57 BL/6 mice by reducing the permeability of BBB, decreasing the expression and activity of MMP-9, and decreasing the inflammatory infiltration. We think that berberine might be a potential therapeutic agent for MS. Public Library of Science 2010-10-19 /pmc/articles/PMC2957444/ /pubmed/20976070 http://dx.doi.org/10.1371/journal.pone.0013489 Text en Ma et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ma, Xiaomeng
Jiang, Ying
Wu, Aimin
Chen, Xiaohong
Pi, Rongbiao
Liu, Mei
Liu, Yingying
Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice
title Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice
title_full Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice
title_fullStr Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice
title_full_unstemmed Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice
title_short Berberine Attenuates Experimental Autoimmune Encephalomyelitis in C57 BL/6 Mice
title_sort berberine attenuates experimental autoimmune encephalomyelitis in c57 bl/6 mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957444/
https://www.ncbi.nlm.nih.gov/pubmed/20976070
http://dx.doi.org/10.1371/journal.pone.0013489
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