The Structure and Mechanism of the Mycobacterium tuberculosis Cyclodityrosine Synthetase

The Mycobacterium tuberculosis enzyme Rv2275 catalyzes the formation of cyclo(L-Tyr-L-Tyr) using two molecules of Tyr-tRNA(Tyr) as substrates. The three-dimensional structure of Rv2275 was determined to 2.0 Å resolution, revealing that Rv2275 is structurally related to the class Ic aminoacyl-tRNA-sy...

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Detalles Bibliográficos
Autores principales: Vetting, Matthew W., Hegde, Subray S., Blanchard, John S.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957485/
https://www.ncbi.nlm.nih.gov/pubmed/20852636
http://dx.doi.org/10.1038/nchembio.440
Descripción
Sumario:The Mycobacterium tuberculosis enzyme Rv2275 catalyzes the formation of cyclo(L-Tyr-L-Tyr) using two molecules of Tyr-tRNA(Tyr) as substrates. The three-dimensional structure of Rv2275 was determined to 2.0 Å resolution, revealing that Rv2275 is structurally related to the class Ic aminoacyl-tRNA-synthetase family of enzymes. Mutagenesis and radioactive labeling suggests a covalent intermediate in which L-tyrosine is transferred from Tyr-tRNA(Tyr) to an active site serine (S88) by transesterification and with E233 serving as a critical base catalyzing dipeptide bond formation.

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