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Effects of macromolecular crowding on intracellular diffusion from a single particle perspective
Compared to biochemical reactions taking place in relatively well-defined aqueous solutions in vitro, the corresponding reactions happening in vivo occur in extremely complex environments containing only 60–70% water by volume, with the remainder consisting of an undefined array of bio-molecules. In...
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Formato: | Texto |
Lenguaje: | English |
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Springer-Verlag
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957576/ https://www.ncbi.nlm.nih.gov/pubmed/21088688 http://dx.doi.org/10.1007/s12551-010-0029-0 |
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author | Hall, Damien Hoshino, Masaru |
author_facet | Hall, Damien Hoshino, Masaru |
author_sort | Hall, Damien |
collection | PubMed |
description | Compared to biochemical reactions taking place in relatively well-defined aqueous solutions in vitro, the corresponding reactions happening in vivo occur in extremely complex environments containing only 60–70% water by volume, with the remainder consisting of an undefined array of bio-molecules. In a biological setting, such extremely complex and volume-occupied solution environments are termed ‘crowded’. Through a range of intermolecular forces and pseudo-forces, this complex background environment may cause biochemical reactions to behave differently to their in vitro counterparts. In this review, we seek to highlight how the complex background environment of the cell can affect the diffusion of substances within it. Engaging the subject from the perspective of a single particle’s motion, we place the focus of our review on two areas: (1) experimental procedures for conducting single particle tracking experiments within cells along with methods for extracting information from these experiments; (2) theoretical factors affecting the translational diffusion of single molecules within crowded two-dimensional membrane and three-dimensional solution environments. We conclude by discussing a number of recent publications relating to intracellular diffusion in light of the reviewed material. |
format | Text |
id | pubmed-2957576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-29575762010-11-16 Effects of macromolecular crowding on intracellular diffusion from a single particle perspective Hall, Damien Hoshino, Masaru Biophys Rev Review Compared to biochemical reactions taking place in relatively well-defined aqueous solutions in vitro, the corresponding reactions happening in vivo occur in extremely complex environments containing only 60–70% water by volume, with the remainder consisting of an undefined array of bio-molecules. In a biological setting, such extremely complex and volume-occupied solution environments are termed ‘crowded’. Through a range of intermolecular forces and pseudo-forces, this complex background environment may cause biochemical reactions to behave differently to their in vitro counterparts. In this review, we seek to highlight how the complex background environment of the cell can affect the diffusion of substances within it. Engaging the subject from the perspective of a single particle’s motion, we place the focus of our review on two areas: (1) experimental procedures for conducting single particle tracking experiments within cells along with methods for extracting information from these experiments; (2) theoretical factors affecting the translational diffusion of single molecules within crowded two-dimensional membrane and three-dimensional solution environments. We conclude by discussing a number of recent publications relating to intracellular diffusion in light of the reviewed material. Springer-Verlag 2010-02-06 /pmc/articles/PMC2957576/ /pubmed/21088688 http://dx.doi.org/10.1007/s12551-010-0029-0 Text en © International Union for Pure and Applied Biophysics (IUPAB) and Springer 2010 |
spellingShingle | Review Hall, Damien Hoshino, Masaru Effects of macromolecular crowding on intracellular diffusion from a single particle perspective |
title | Effects of macromolecular crowding on intracellular diffusion from a single particle perspective |
title_full | Effects of macromolecular crowding on intracellular diffusion from a single particle perspective |
title_fullStr | Effects of macromolecular crowding on intracellular diffusion from a single particle perspective |
title_full_unstemmed | Effects of macromolecular crowding on intracellular diffusion from a single particle perspective |
title_short | Effects of macromolecular crowding on intracellular diffusion from a single particle perspective |
title_sort | effects of macromolecular crowding on intracellular diffusion from a single particle perspective |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957576/ https://www.ncbi.nlm.nih.gov/pubmed/21088688 http://dx.doi.org/10.1007/s12551-010-0029-0 |
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