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Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals

A new flow procedure based on multicommutation with chemiluminometric detection was developed to quantify gentamicin sulphate in pharmaceutical formulations. This approach is based on gentamicin's ability to inhibit the chemiluminometric reaction between luminol and hypochlorite in alkaline med...

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Autores principales: Santos, Lúcia H. M. L. M., Araújo, A. N., Reis, Boaventura, Montenegro, M. C. B. S. M.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957854/
https://www.ncbi.nlm.nih.gov/pubmed/20981343
http://dx.doi.org/10.1155/2010/913207
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author Santos, Lúcia H. M. L. M.
Araújo, A. N.
Reis, Boaventura
Montenegro, M. C. B. S. M.
author_facet Santos, Lúcia H. M. L. M.
Araújo, A. N.
Reis, Boaventura
Montenegro, M. C. B. S. M.
author_sort Santos, Lúcia H. M. L. M.
collection PubMed
description A new flow procedure based on multicommutation with chemiluminometric detection was developed to quantify gentamicin sulphate in pharmaceutical formulations. This approach is based on gentamicin's ability to inhibit the chemiluminometric reaction between luminol and hypochlorite in alkaline medium, causing a decrease in the analytical signal. The inhibition of the analytical signal is proportional to the concentration of gentamicin sulphate, within a linear range of 1 to 4 μg mL(−1) with a coefficient variation <3%. A sample throughput of 55 samples h(−1) was obtained. The developed method is sensitive, simple, with low reagent consumption, reproducible, and inexpensive, and when applied to the analysis of pharmaceutical formulations (eye drops and injections) it gave results with RSD between 1.10 and 4.40%.
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spelling pubmed-29578542010-10-27 Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals Santos, Lúcia H. M. L. M. Araújo, A. N. Reis, Boaventura Montenegro, M. C. B. S. M. J Autom Methods Manag Chem Research Article A new flow procedure based on multicommutation with chemiluminometric detection was developed to quantify gentamicin sulphate in pharmaceutical formulations. This approach is based on gentamicin's ability to inhibit the chemiluminometric reaction between luminol and hypochlorite in alkaline medium, causing a decrease in the analytical signal. The inhibition of the analytical signal is proportional to the concentration of gentamicin sulphate, within a linear range of 1 to 4 μg mL(−1) with a coefficient variation <3%. A sample throughput of 55 samples h(−1) was obtained. The developed method is sensitive, simple, with low reagent consumption, reproducible, and inexpensive, and when applied to the analysis of pharmaceutical formulations (eye drops and injections) it gave results with RSD between 1.10 and 4.40%. Hindawi Publishing Corporation 2010 2010-10-13 /pmc/articles/PMC2957854/ /pubmed/20981343 http://dx.doi.org/10.1155/2010/913207 Text en Copyright © 2010 Lúcia H. M. L. M. Santos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Santos, Lúcia H. M. L. M.
Araújo, A. N.
Reis, Boaventura
Montenegro, M. C. B. S. M.
Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals
title Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals
title_full Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals
title_fullStr Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals
title_full_unstemmed Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals
title_short Development of a Multicommutated Flow System with Chemiluminometric Detection for Quantification of Gentamicin in Pharmaceuticals
title_sort development of a multicommutated flow system with chemiluminometric detection for quantification of gentamicin in pharmaceuticals
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957854/
https://www.ncbi.nlm.nih.gov/pubmed/20981343
http://dx.doi.org/10.1155/2010/913207
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