Cargando…
Atrazine Binds to the Growth Hormone–Releasing Hormone Receptor and Affects Growth Hormone Gene Expression
BACKGROUND: Atrazine (ATR), a commonly used herbicide in the United States, is widely distributed in water and soil because of its mobility through ecosystems and its persistence in the environment. ATR has been associated with defects in sexual development in animals, but studies on mammalian syste...
Autores principales: | , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
National Institute of Environmental Health Sciences
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957919/ https://www.ncbi.nlm.nih.gov/pubmed/20529762 http://dx.doi.org/10.1289/ehp.0900738 |
_version_ | 1782188268113100800 |
---|---|
author | Fakhouri, Walid D. Nuñez, Joseph L. Trail, Frances |
author_facet | Fakhouri, Walid D. Nuñez, Joseph L. Trail, Frances |
author_sort | Fakhouri, Walid D. |
collection | PubMed |
description | BACKGROUND: Atrazine (ATR), a commonly used herbicide in the United States, is widely distributed in water and soil because of its mobility through ecosystems and its persistence in the environment. ATR has been associated with defects in sexual development in animals, but studies on mammalian systems have failed to clearly identify a cellular target. OBJECTIVES: Our goal in this study was to identify a ligand-binding receptor for ATR in pituitary cells that may explain the mechanism of action at the gene expression level. METHODS: We used pituitary cells from postnatal day 7 male rats and pituitary cell lines to study the effect of ATR on gene expression of growth hormone (GH), luteinizing hormone (LH), and prolactin (PRL) at RNA and protein levels. (14)C-ATR was used to determine its specific binding to the growth hormone–releasing hormone receptor (GHRHR). The effect of ATR on structural proteins was visualized using immunofluorescent in situ staining. RESULTS: The treatment of rat pituitary cells with ATR, at environmentally relevant concentrations (1 ppb and 1 ppm), resulted in a reduction of GH expression. This effect appeared to result from the inhibition of GH gene transcription due to ATR binding to the GHRHR of the pituitary cells. CONCLUSIONS: Identification of GHRHR as the target of ATR is consistent with the myriad effects previously reported for ATR in mammalian systems. These findings may lead to a better understanding of the hazards of environmental ATR contamination and inform efforts to develop guidelines for establishing safe levels in water systems. |
format | Text |
id | pubmed-2957919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | National Institute of Environmental Health Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-29579192010-10-21 Atrazine Binds to the Growth Hormone–Releasing Hormone Receptor and Affects Growth Hormone Gene Expression Fakhouri, Walid D. Nuñez, Joseph L. Trail, Frances Environ Health Perspect Research BACKGROUND: Atrazine (ATR), a commonly used herbicide in the United States, is widely distributed in water and soil because of its mobility through ecosystems and its persistence in the environment. ATR has been associated with defects in sexual development in animals, but studies on mammalian systems have failed to clearly identify a cellular target. OBJECTIVES: Our goal in this study was to identify a ligand-binding receptor for ATR in pituitary cells that may explain the mechanism of action at the gene expression level. METHODS: We used pituitary cells from postnatal day 7 male rats and pituitary cell lines to study the effect of ATR on gene expression of growth hormone (GH), luteinizing hormone (LH), and prolactin (PRL) at RNA and protein levels. (14)C-ATR was used to determine its specific binding to the growth hormone–releasing hormone receptor (GHRHR). The effect of ATR on structural proteins was visualized using immunofluorescent in situ staining. RESULTS: The treatment of rat pituitary cells with ATR, at environmentally relevant concentrations (1 ppb and 1 ppm), resulted in a reduction of GH expression. This effect appeared to result from the inhibition of GH gene transcription due to ATR binding to the GHRHR of the pituitary cells. CONCLUSIONS: Identification of GHRHR as the target of ATR is consistent with the myriad effects previously reported for ATR in mammalian systems. These findings may lead to a better understanding of the hazards of environmental ATR contamination and inform efforts to develop guidelines for establishing safe levels in water systems. National Institute of Environmental Health Sciences 2010-10 2010-06-08 /pmc/articles/PMC2957919/ /pubmed/20529762 http://dx.doi.org/10.1289/ehp.0900738 Text en http://creativecommons.org/publicdomain/mark/1.0/ Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, ?Reproduced with permission from Environmental Health Perspectives?); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright. |
spellingShingle | Research Fakhouri, Walid D. Nuñez, Joseph L. Trail, Frances Atrazine Binds to the Growth Hormone–Releasing Hormone Receptor and Affects Growth Hormone Gene Expression |
title | Atrazine Binds to the Growth Hormone–Releasing Hormone Receptor and Affects Growth Hormone Gene Expression |
title_full | Atrazine Binds to the Growth Hormone–Releasing Hormone Receptor and Affects Growth Hormone Gene Expression |
title_fullStr | Atrazine Binds to the Growth Hormone–Releasing Hormone Receptor and Affects Growth Hormone Gene Expression |
title_full_unstemmed | Atrazine Binds to the Growth Hormone–Releasing Hormone Receptor and Affects Growth Hormone Gene Expression |
title_short | Atrazine Binds to the Growth Hormone–Releasing Hormone Receptor and Affects Growth Hormone Gene Expression |
title_sort | atrazine binds to the growth hormone–releasing hormone receptor and affects growth hormone gene expression |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957919/ https://www.ncbi.nlm.nih.gov/pubmed/20529762 http://dx.doi.org/10.1289/ehp.0900738 |
work_keys_str_mv | AT fakhouriwalidd atrazinebindstothegrowthhormonereleasinghormonereceptorandaffectsgrowthhormonegeneexpression AT nunezjosephl atrazinebindstothegrowthhormonereleasinghormonereceptorandaffectsgrowthhormonegeneexpression AT trailfrances atrazinebindstothegrowthhormonereleasinghormonereceptorandaffectsgrowthhormonegeneexpression |