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Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer

BACKGROUND: at present, pathogenesis of bladder cancer (BC) has not been fully elucidated. Aim of this study is to investigate the role of human telomerase RNA (hTR), human telomerase reverse transcriptase (hTERT) and CDC28 protein kinase regulatory subunit 2 (CKS2) in bladder carcinogenesis and the...

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Autores principales: Mezzasoma, Letizia, Antognelli, Cinzia, Del Buono, Chiara, Stracci, Fabrizio, Cottini, Emanuele, Cochetti, Giovanni, Talesa, Vincenzo N, Mearini, Ettore
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2959011/
https://www.ncbi.nlm.nih.gov/pubmed/20920335
http://dx.doi.org/10.1186/1471-2490-10-17
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author Mezzasoma, Letizia
Antognelli, Cinzia
Del Buono, Chiara
Stracci, Fabrizio
Cottini, Emanuele
Cochetti, Giovanni
Talesa, Vincenzo N
Mearini, Ettore
author_facet Mezzasoma, Letizia
Antognelli, Cinzia
Del Buono, Chiara
Stracci, Fabrizio
Cottini, Emanuele
Cochetti, Giovanni
Talesa, Vincenzo N
Mearini, Ettore
author_sort Mezzasoma, Letizia
collection PubMed
description BACKGROUND: at present, pathogenesis of bladder cancer (BC) has not been fully elucidated. Aim of this study is to investigate the role of human telomerase RNA (hTR), human telomerase reverse transcriptase (hTERT) and CDC28 protein kinase regulatory subunit 2 (CKS2) in bladder carcinogenesis and their possible clinical significance; METHODS: the transcript levels of hTR, hTERT and CKS2 were quantified by Real time reverse transcriptase chain reaction in exfoliated cells from bladder washings of 36 patients with BC and 58 controls. The statistical significance of differences between BC bearing patients and control groups, in the general as well as in the stratified analysis (superficial or invasive BC), was assessed by Student's t test. Non parametric Receiver Operating Characteristics analysis (ROC) was performed to ascertain the accuracy of study variables to discriminate between BC and controls. The clinical value of concomitant examination of hTR, hTERT and CKS2 was evaluated by logistic regression analysis; RESULTS: a significant decrease in hTR and a significant increase in hTERT or CKS2 gene expression were found between BC bearing patients and controls, as well as in the subgroups analysis. The area under the curve (AUC) indicated an average discrimination power for the three genes, both in the general and subgroups analysis, when singularly considered. The ability to significantly discriminate between superficial and invasive BC was observed only for hTR transcript levels. A combined model including hTR and CKS2 was the best one in BC diagnosis; CONCLUSIONS: our results, obtained from a sample set particularly rich of exfoliated cells, provide further molecular evidence on the involvement of hTR, hTERT and CKS2 gene expression in BC carcinogenesis. In particular, while hTERT and CKS2 gene expression seems to have a major involvement in the early stages of the disease, hTR gene expression, seems to be more involved in progression. In addition, our findings suggest that the studied genes have a clinical role in discriminating between BC and controls in the general as well as in the stratified analysis, when singularly considered. A combined model improved over the single marker BC diagnosis.
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spelling pubmed-29590112010-10-22 Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer Mezzasoma, Letizia Antognelli, Cinzia Del Buono, Chiara Stracci, Fabrizio Cottini, Emanuele Cochetti, Giovanni Talesa, Vincenzo N Mearini, Ettore BMC Urol Research Article BACKGROUND: at present, pathogenesis of bladder cancer (BC) has not been fully elucidated. Aim of this study is to investigate the role of human telomerase RNA (hTR), human telomerase reverse transcriptase (hTERT) and CDC28 protein kinase regulatory subunit 2 (CKS2) in bladder carcinogenesis and their possible clinical significance; METHODS: the transcript levels of hTR, hTERT and CKS2 were quantified by Real time reverse transcriptase chain reaction in exfoliated cells from bladder washings of 36 patients with BC and 58 controls. The statistical significance of differences between BC bearing patients and control groups, in the general as well as in the stratified analysis (superficial or invasive BC), was assessed by Student's t test. Non parametric Receiver Operating Characteristics analysis (ROC) was performed to ascertain the accuracy of study variables to discriminate between BC and controls. The clinical value of concomitant examination of hTR, hTERT and CKS2 was evaluated by logistic regression analysis; RESULTS: a significant decrease in hTR and a significant increase in hTERT or CKS2 gene expression were found between BC bearing patients and controls, as well as in the subgroups analysis. The area under the curve (AUC) indicated an average discrimination power for the three genes, both in the general and subgroups analysis, when singularly considered. The ability to significantly discriminate between superficial and invasive BC was observed only for hTR transcript levels. A combined model including hTR and CKS2 was the best one in BC diagnosis; CONCLUSIONS: our results, obtained from a sample set particularly rich of exfoliated cells, provide further molecular evidence on the involvement of hTR, hTERT and CKS2 gene expression in BC carcinogenesis. In particular, while hTERT and CKS2 gene expression seems to have a major involvement in the early stages of the disease, hTR gene expression, seems to be more involved in progression. In addition, our findings suggest that the studied genes have a clinical role in discriminating between BC and controls in the general as well as in the stratified analysis, when singularly considered. A combined model improved over the single marker BC diagnosis. BioMed Central 2010-10-04 /pmc/articles/PMC2959011/ /pubmed/20920335 http://dx.doi.org/10.1186/1471-2490-10-17 Text en Copyright ©2010 Mezzasoma et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mezzasoma, Letizia
Antognelli, Cinzia
Del Buono, Chiara
Stracci, Fabrizio
Cottini, Emanuele
Cochetti, Giovanni
Talesa, Vincenzo N
Mearini, Ettore
Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer
title Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer
title_full Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer
title_fullStr Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer
title_full_unstemmed Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer
title_short Expression and biological-clinical significance of hTR, hTERT and CKS2 in washing fluids of patients with bladder cancer
title_sort expression and biological-clinical significance of htr, htert and cks2 in washing fluids of patients with bladder cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2959011/
https://www.ncbi.nlm.nih.gov/pubmed/20920335
http://dx.doi.org/10.1186/1471-2490-10-17
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