Cargando…

Ca(2+)-Mg(2+)-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session

In the course of chronic kidney disease (CKD) the intracellular erythrocyte calcium (Ca(i)(2+)) level increases along with the progression of the disease. The decreased activity of Ca(2+)-Mg(2+)-dependent ATP-ase (PMCA) and its endogenous modulators calmodulin (CALM), calpain (CANP), and calpastatin...

Descripción completa

Detalles Bibliográficos
Autores principales: Polak-Jonkisz, Dorota, Purzyc, Leszek, Zwolińska, Danuta
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962792/
https://www.ncbi.nlm.nih.gov/pubmed/20882298
http://dx.doi.org/10.1007/s00467-010-1634-7
_version_ 1782189211567259648
author Polak-Jonkisz, Dorota
Purzyc, Leszek
Zwolińska, Danuta
author_facet Polak-Jonkisz, Dorota
Purzyc, Leszek
Zwolińska, Danuta
author_sort Polak-Jonkisz, Dorota
collection PubMed
description In the course of chronic kidney disease (CKD) the intracellular erythrocyte calcium (Ca(i)(2+)) level increases along with the progression of the disease. The decreased activity of Ca(2+)-Mg(2+)-dependent ATP-ase (PMCA) and its endogenous modulators calmodulin (CALM), calpain (CANP), and calpastatin (CAST) are all responsible for disturbed calcium metabolism. The aim of the study was to analyze the activity of PMCA, CALM, and the CANP-CAST system in the red blood cells (RBCs) of hemodialyzed (HD) children and to estimate the impact of a single HD session on the aforementioned disturbances. Eighteen patients on maintenance HD and 30 healthy subjects were included in the study. CALM, Ca(i)(2+) levels and basal PMCA (bPMCA), PMCA, CANP, and CAST activities were determined in RBCs before HD, after HD, and before the next HD session. Prior to the HD session, the level of Ca(i)(2+) and the CAST activity were significantly higher, whereas bPMCA, PMCA, and CANP activities and the CALM level were significantly lower than in controls. After the HD session, the Ca(i)(2+) concentration and the CAST activity significantly decreased compared with the basal values, whereas the other parameters significantly increased, although they did not reach the levels of healthy children. The values observed prior to both HD sessions were similar. Ca(i)(2+) homeostasis is severely disturbed in HD children, which may be caused by the reduction in the PMCA activity, CALM deficiency, and CANP-CAST system disturbances. A single HD session improved these disturbances but the effect is transient.
format Text
id pubmed-2962792
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-29627922010-11-16 Ca(2+)-Mg(2+)-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session Polak-Jonkisz, Dorota Purzyc, Leszek Zwolińska, Danuta Pediatr Nephrol Original Article In the course of chronic kidney disease (CKD) the intracellular erythrocyte calcium (Ca(i)(2+)) level increases along with the progression of the disease. The decreased activity of Ca(2+)-Mg(2+)-dependent ATP-ase (PMCA) and its endogenous modulators calmodulin (CALM), calpain (CANP), and calpastatin (CAST) are all responsible for disturbed calcium metabolism. The aim of the study was to analyze the activity of PMCA, CALM, and the CANP-CAST system in the red blood cells (RBCs) of hemodialyzed (HD) children and to estimate the impact of a single HD session on the aforementioned disturbances. Eighteen patients on maintenance HD and 30 healthy subjects were included in the study. CALM, Ca(i)(2+) levels and basal PMCA (bPMCA), PMCA, CANP, and CAST activities were determined in RBCs before HD, after HD, and before the next HD session. Prior to the HD session, the level of Ca(i)(2+) and the CAST activity were significantly higher, whereas bPMCA, PMCA, and CANP activities and the CALM level were significantly lower than in controls. After the HD session, the Ca(i)(2+) concentration and the CAST activity significantly decreased compared with the basal values, whereas the other parameters significantly increased, although they did not reach the levels of healthy children. The values observed prior to both HD sessions were similar. Ca(i)(2+) homeostasis is severely disturbed in HD children, which may be caused by the reduction in the PMCA activity, CALM deficiency, and CANP-CAST system disturbances. A single HD session improved these disturbances but the effect is transient. Springer-Verlag 2010-09-30 2010 /pmc/articles/PMC2962792/ /pubmed/20882298 http://dx.doi.org/10.1007/s00467-010-1634-7 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
Polak-Jonkisz, Dorota
Purzyc, Leszek
Zwolińska, Danuta
Ca(2+)-Mg(2+)-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session
title Ca(2+)-Mg(2+)-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session
title_full Ca(2+)-Mg(2+)-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session
title_fullStr Ca(2+)-Mg(2+)-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session
title_full_unstemmed Ca(2+)-Mg(2+)-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session
title_short Ca(2+)-Mg(2+)-dependent ATP-ase activity in hemodialyzed children. Effect of a hemodialysis session
title_sort ca(2+)-mg(2+)-dependent atp-ase activity in hemodialyzed children. effect of a hemodialysis session
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962792/
https://www.ncbi.nlm.nih.gov/pubmed/20882298
http://dx.doi.org/10.1007/s00467-010-1634-7
work_keys_str_mv AT polakjonkiszdorota ca2mg2dependentatpaseactivityinhemodialyzedchildreneffectofahemodialysissession
AT purzycleszek ca2mg2dependentatpaseactivityinhemodialyzedchildreneffectofahemodialysissession
AT zwolinskadanuta ca2mg2dependentatpaseactivityinhemodialyzedchildreneffectofahemodialysissession