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Signal enhancement in protein NMR using the spin-noise tuning optimum
We have assessed the potential of an alternative probe tuning strategy based on the spin-noise response for application in common high-resolution multi-dimensional biomolecular NMR experiments with water signal suppression on aqueous and salty samples. The method requires the adjustment of the optim...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962798/ https://www.ncbi.nlm.nih.gov/pubmed/20924647 http://dx.doi.org/10.1007/s10858-010-9446-4 |
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author | Nausner, Martin Goger, Michael Bendet-Taicher, Eli Schlagnitweit, Judith Jerschow, Alexej Müller, Norbert |
author_facet | Nausner, Martin Goger, Michael Bendet-Taicher, Eli Schlagnitweit, Judith Jerschow, Alexej Müller, Norbert |
author_sort | Nausner, Martin |
collection | PubMed |
description | We have assessed the potential of an alternative probe tuning strategy based on the spin-noise response for application in common high-resolution multi-dimensional biomolecular NMR experiments with water signal suppression on aqueous and salty samples. The method requires the adjustment of the optimal tuning condition, which may be offset by several 100 kHz from the conventional tuning settings using the noise response of the water protons as an indicator. Although the radio frequency-pulse durations are typically longer under such conditions, signal-to-noise gains of up to 22% were achieved. At salt concentrations up to 100 mM a substantial sensitivity gain was observed. |
format | Text |
id | pubmed-2962798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-29627982010-11-16 Signal enhancement in protein NMR using the spin-noise tuning optimum Nausner, Martin Goger, Michael Bendet-Taicher, Eli Schlagnitweit, Judith Jerschow, Alexej Müller, Norbert J Biomol NMR Article We have assessed the potential of an alternative probe tuning strategy based on the spin-noise response for application in common high-resolution multi-dimensional biomolecular NMR experiments with water signal suppression on aqueous and salty samples. The method requires the adjustment of the optimal tuning condition, which may be offset by several 100 kHz from the conventional tuning settings using the noise response of the water protons as an indicator. Although the radio frequency-pulse durations are typically longer under such conditions, signal-to-noise gains of up to 22% were achieved. At salt concentrations up to 100 mM a substantial sensitivity gain was observed. Springer Netherlands 2010-10-06 2010 /pmc/articles/PMC2962798/ /pubmed/20924647 http://dx.doi.org/10.1007/s10858-010-9446-4 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Nausner, Martin Goger, Michael Bendet-Taicher, Eli Schlagnitweit, Judith Jerschow, Alexej Müller, Norbert Signal enhancement in protein NMR using the spin-noise tuning optimum |
title | Signal enhancement in protein NMR using the spin-noise tuning optimum |
title_full | Signal enhancement in protein NMR using the spin-noise tuning optimum |
title_fullStr | Signal enhancement in protein NMR using the spin-noise tuning optimum |
title_full_unstemmed | Signal enhancement in protein NMR using the spin-noise tuning optimum |
title_short | Signal enhancement in protein NMR using the spin-noise tuning optimum |
title_sort | signal enhancement in protein nmr using the spin-noise tuning optimum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962798/ https://www.ncbi.nlm.nih.gov/pubmed/20924647 http://dx.doi.org/10.1007/s10858-010-9446-4 |
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