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The –SH Protection Method for Determining Accurate K(d) Values for Enzyme-Coenzyme Complexes of NAD(+)-Dependent Glutamate Dehydrogenase and Engineered Mutants: Evidence for Nonproductive NADPH Complexes

Inactivation rates have been measured for clostridial glutamate dehydrogenase and several engineered mutants at various DTNB concentrations. Analysis of rate constants allowed determination of K(d) for each non-covalent enzyme-DTNB complex and the rate constant for reaction to form the inactive enzy...

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Detalles Bibliográficos
Autores principales:	Griffin, Joanna, Engel, Paul C.
Formato:	Texto
Lenguaje:	English
Publicado:	SAGE-Hindawi Access to Research 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962915/ https://www.ncbi.nlm.nih.gov/pubmed/21048875 http://dx.doi.org/10.4061/2010/951472

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