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Alteration in Marrow Stromal Microenvironment and Apoptosis Mechanisms Involved in Aplastic Anemia: An Animal Model to Study the Possible Disease Pathology

Aplastic anemia (AA) is a heterogeneous disorder of bone marrow failure syndrome. Suggested mechanisms include a primary stem cell deficiency or defect, a secondary stem cell defect due to abnormal regulation between cell death and differentiation, or a deficient microenvironment. In this study, we...

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Autores principales: Chatterjee, Sumanta, Dutta, Ranjan Kumar, Basak, Pratima, Das, Prosun, Das, Madhurima, Pereira, Jacintha Archana, Chaklader, Malay, Chaudhuri, Samaresh, Law, Sujata
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2963319/
https://www.ncbi.nlm.nih.gov/pubmed/21048856
http://dx.doi.org/10.4061/2010/932354
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author Chatterjee, Sumanta
Dutta, Ranjan Kumar
Basak, Pratima
Das, Prosun
Das, Madhurima
Pereira, Jacintha Archana
Chaklader, Malay
Chaudhuri, Samaresh
Law, Sujata
author_facet Chatterjee, Sumanta
Dutta, Ranjan Kumar
Basak, Pratima
Das, Prosun
Das, Madhurima
Pereira, Jacintha Archana
Chaklader, Malay
Chaudhuri, Samaresh
Law, Sujata
author_sort Chatterjee, Sumanta
collection PubMed
description Aplastic anemia (AA) is a heterogeneous disorder of bone marrow failure syndrome. Suggested mechanisms include a primary stem cell deficiency or defect, a secondary stem cell defect due to abnormal regulation between cell death and differentiation, or a deficient microenvironment. In this study, we have tried to investigate the alterations in hematopoietic microenvironment and underlying mechanisms involved in such alterations in an animal model of drug induced AA. We presented the results of studying long term marrow culture, marrow ultra-structure, marrow adherent and hematopoietic progenitor cell colony formation, flowcytometric analysis of marrow stem and stromal progenitor populations and apoptosis mechanism involved in aplastic anemia. The AA marrow showed impairment in cellular proliferation and maturation and failed to generate a functional stromal microenvironment even after 19 days of culture. Ultra-structural analysis showed a degenerated and deformed marrow cellular association in AA. Colony forming units (CFUs) were also severely reduced in AA. Significantly decreased marrow stem and stromal progenitor population with subsequently increased expression levels of both the extracellular and intracellular apoptosis inducer markers in the AA marrow cells essentially pointed towards the defective hematopoiesis; moreover, a deficient and apoptotic microenvironment and the microenvironmental components might have played the important role in the possible pathogenesis of AA.
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spelling pubmed-29633192010-11-03 Alteration in Marrow Stromal Microenvironment and Apoptosis Mechanisms Involved in Aplastic Anemia: An Animal Model to Study the Possible Disease Pathology Chatterjee, Sumanta Dutta, Ranjan Kumar Basak, Pratima Das, Prosun Das, Madhurima Pereira, Jacintha Archana Chaklader, Malay Chaudhuri, Samaresh Law, Sujata Stem Cells Int Research Article Aplastic anemia (AA) is a heterogeneous disorder of bone marrow failure syndrome. Suggested mechanisms include a primary stem cell deficiency or defect, a secondary stem cell defect due to abnormal regulation between cell death and differentiation, or a deficient microenvironment. In this study, we have tried to investigate the alterations in hematopoietic microenvironment and underlying mechanisms involved in such alterations in an animal model of drug induced AA. We presented the results of studying long term marrow culture, marrow ultra-structure, marrow adherent and hematopoietic progenitor cell colony formation, flowcytometric analysis of marrow stem and stromal progenitor populations and apoptosis mechanism involved in aplastic anemia. The AA marrow showed impairment in cellular proliferation and maturation and failed to generate a functional stromal microenvironment even after 19 days of culture. Ultra-structural analysis showed a degenerated and deformed marrow cellular association in AA. Colony forming units (CFUs) were also severely reduced in AA. Significantly decreased marrow stem and stromal progenitor population with subsequently increased expression levels of both the extracellular and intracellular apoptosis inducer markers in the AA marrow cells essentially pointed towards the defective hematopoiesis; moreover, a deficient and apoptotic microenvironment and the microenvironmental components might have played the important role in the possible pathogenesis of AA. SAGE-Hindawi Access to Research 2010-09-19 /pmc/articles/PMC2963319/ /pubmed/21048856 http://dx.doi.org/10.4061/2010/932354 Text en Copyright © 2010 Sumanta Chatterjee et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chatterjee, Sumanta
Dutta, Ranjan Kumar
Basak, Pratima
Das, Prosun
Das, Madhurima
Pereira, Jacintha Archana
Chaklader, Malay
Chaudhuri, Samaresh
Law, Sujata
Alteration in Marrow Stromal Microenvironment and Apoptosis Mechanisms Involved in Aplastic Anemia: An Animal Model to Study the Possible Disease Pathology
title Alteration in Marrow Stromal Microenvironment and Apoptosis Mechanisms Involved in Aplastic Anemia: An Animal Model to Study the Possible Disease Pathology
title_full Alteration in Marrow Stromal Microenvironment and Apoptosis Mechanisms Involved in Aplastic Anemia: An Animal Model to Study the Possible Disease Pathology
title_fullStr Alteration in Marrow Stromal Microenvironment and Apoptosis Mechanisms Involved in Aplastic Anemia: An Animal Model to Study the Possible Disease Pathology
title_full_unstemmed Alteration in Marrow Stromal Microenvironment and Apoptosis Mechanisms Involved in Aplastic Anemia: An Animal Model to Study the Possible Disease Pathology
title_short Alteration in Marrow Stromal Microenvironment and Apoptosis Mechanisms Involved in Aplastic Anemia: An Animal Model to Study the Possible Disease Pathology
title_sort alteration in marrow stromal microenvironment and apoptosis mechanisms involved in aplastic anemia: an animal model to study the possible disease pathology
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2963319/
https://www.ncbi.nlm.nih.gov/pubmed/21048856
http://dx.doi.org/10.4061/2010/932354
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