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Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells

The spatial organization of peptidoglycan, the major constituent of bacterial cell-walls, is an important, yet still unsolved issue in microbiology. In this paper, we show that the combined use of atomic force microscopy and cell wall mutants is a powerful platform for probing the nanoscale architec...

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Autores principales: Andre, Guillaume, Kulakauskas, Saulius, Chapot-Chartier, Marie-Pierre, Navet, Benjamine, Deghorain, Marie, Bernard, Elvis, Hols, Pascal, Dufrêne, Yves F.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964452/
https://www.ncbi.nlm.nih.gov/pubmed/20975688
http://dx.doi.org/10.1038/ncomms1027
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author Andre, Guillaume
Kulakauskas, Saulius
Chapot-Chartier, Marie-Pierre
Navet, Benjamine
Deghorain, Marie
Bernard, Elvis
Hols, Pascal
Dufrêne, Yves F.
author_facet Andre, Guillaume
Kulakauskas, Saulius
Chapot-Chartier, Marie-Pierre
Navet, Benjamine
Deghorain, Marie
Bernard, Elvis
Hols, Pascal
Dufrêne, Yves F.
author_sort Andre, Guillaume
collection PubMed
description The spatial organization of peptidoglycan, the major constituent of bacterial cell-walls, is an important, yet still unsolved issue in microbiology. In this paper, we show that the combined use of atomic force microscopy and cell wall mutants is a powerful platform for probing the nanoscale architecture of cell wall peptidoglycan in living Gram-positive bacteria. Using topographic imaging, we found that Lactococcus lactis wild-type cells display a smooth, featureless surface morphology, whereas mutant strains lacking cell wall exopolysaccharides feature 25-nm-wide periodic bands running parallel to the short axis of the cell. In addition, we used single-molecule recognition imaging to show that parallel bands are made of peptidoglycan. Our data, obtained for the first time on living ovococci, argue for an architectural feature of the cell wall in the plane perpendicular to the long axis of the cell. The non-invasive live cell experiments presented here open new avenues for understanding the architecture and assembly of peptidoglycan in Gram-positive bacteria.
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spelling pubmed-29644522010-11-05 Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells Andre, Guillaume Kulakauskas, Saulius Chapot-Chartier, Marie-Pierre Navet, Benjamine Deghorain, Marie Bernard, Elvis Hols, Pascal Dufrêne, Yves F. Nat Commun Article The spatial organization of peptidoglycan, the major constituent of bacterial cell-walls, is an important, yet still unsolved issue in microbiology. In this paper, we show that the combined use of atomic force microscopy and cell wall mutants is a powerful platform for probing the nanoscale architecture of cell wall peptidoglycan in living Gram-positive bacteria. Using topographic imaging, we found that Lactococcus lactis wild-type cells display a smooth, featureless surface morphology, whereas mutant strains lacking cell wall exopolysaccharides feature 25-nm-wide periodic bands running parallel to the short axis of the cell. In addition, we used single-molecule recognition imaging to show that parallel bands are made of peptidoglycan. Our data, obtained for the first time on living ovococci, argue for an architectural feature of the cell wall in the plane perpendicular to the long axis of the cell. The non-invasive live cell experiments presented here open new avenues for understanding the architecture and assembly of peptidoglycan in Gram-positive bacteria. Nature Publishing Group 2010-06-15 /pmc/articles/PMC2964452/ /pubmed/20975688 http://dx.doi.org/10.1038/ncomms1027 Text en Copyright © 2010, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by-nc-sa/3.0/ Th is work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Article
Andre, Guillaume
Kulakauskas, Saulius
Chapot-Chartier, Marie-Pierre
Navet, Benjamine
Deghorain, Marie
Bernard, Elvis
Hols, Pascal
Dufrêne, Yves F.
Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells
title Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells
title_full Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells
title_fullStr Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells
title_full_unstemmed Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells
title_short Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells
title_sort imaging the nanoscale organization of peptidoglycan in living lactococcus lactis cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964452/
https://www.ncbi.nlm.nih.gov/pubmed/20975688
http://dx.doi.org/10.1038/ncomms1027
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