Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors

An amphiphilic disulfide-containing polyamidoamine was synthesized by Michael-type polyaddition reaction of piperazine to equimolar N, N′-bis(acryloyl)cystamine with 90% yield. The polycationic micelles (198 nm, 32.5 mV), prepared from the amphiphilic polyamidoamine by dialysis method, can condense...

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Detalles Bibliográficos
Autores principales: Zhang, Min, Xue, Ya-Nan, Liu, Min, Zhuo, Ren-Xi, Huang, Shi-Wen
Formato: Texto
Lenguaje:English
Publicado: Springer 2010
Materias:
Nano Express
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964480/
https://www.ncbi.nlm.nih.gov/pubmed/21124632
http://dx.doi.org/10.1007/s11671-010-9716-9
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author Zhang, Min
Xue, Ya-Nan
Liu, Min
Zhuo, Ren-Xi
Huang, Shi-Wen
author_facet Zhang, Min
Xue, Ya-Nan
Liu, Min
Zhuo, Ren-Xi
Huang, Shi-Wen
author_sort Zhang, Min
collection PubMed
description An amphiphilic disulfide-containing polyamidoamine was synthesized by Michael-type polyaddition reaction of piperazine to equimolar N, N′-bis(acryloyl)cystamine with 90% yield. The polycationic micelles (198 nm, 32.5 mV), prepared from the amphiphilic polyamidoamine by dialysis method, can condense foreign plasmid DNA to form nanosized polycationic micelles/DNA polyelectrolyte complexes with positive charges, which transfected 293T cells with high efficiency. Under optimized conditions, the transfection efficiencies of polycationic micelles/DNA complexes are comparable to, or even higher than that of commercially available branched PEI (Mw 25 kDa).
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spelling pubmed-29644802010-11-29 Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors Zhang, Min Xue, Ya-Nan Liu, Min Zhuo, Ren-Xi Huang, Shi-Wen Nanoscale Res Lett Nano Express An amphiphilic disulfide-containing polyamidoamine was synthesized by Michael-type polyaddition reaction of piperazine to equimolar N, N′-bis(acryloyl)cystamine with 90% yield. The polycationic micelles (198 nm, 32.5 mV), prepared from the amphiphilic polyamidoamine by dialysis method, can condense foreign plasmid DNA to form nanosized polycationic micelles/DNA polyelectrolyte complexes with positive charges, which transfected 293T cells with high efficiency. Under optimized conditions, the transfection efficiencies of polycationic micelles/DNA complexes are comparable to, or even higher than that of commercially available branched PEI (Mw 25 kDa). Springer 2010-08-11 /pmc/articles/PMC2964480/ /pubmed/21124632 http://dx.doi.org/10.1007/s11671-010-9716-9 Text en Copyright © 2010 The Author(s) https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Nano Express
Zhang, Min
Xue, Ya-Nan
Liu, Min
Zhuo, Ren-Xi
Huang, Shi-Wen
Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors
title Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors
title_full Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors
title_fullStr Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors
title_full_unstemmed Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors
title_short Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors
title_sort biocleavable polycationic micelles as highly efficient gene delivery vectors
topic Nano Express
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964480/
https://www.ncbi.nlm.nih.gov/pubmed/21124632
http://dx.doi.org/10.1007/s11671-010-9716-9
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