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The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase
BACKGROUND: The extracellular proteome or secretome of symbiotic bacteria like Rhizobium etli is presumed to be a key element of their infection strategy and survival. Rhizobia infect the roots of leguminous plants and establish a mutually beneficial symbiosis. To find out the possible role of secre...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964644/ https://www.ncbi.nlm.nih.gov/pubmed/20942974 http://dx.doi.org/10.1186/1477-5956-8-51 |
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author | Meneses, Niurka Mendoza-Hernández, Guillermo Encarnación, Sergio |
author_facet | Meneses, Niurka Mendoza-Hernández, Guillermo Encarnación, Sergio |
author_sort | Meneses, Niurka |
collection | PubMed |
description | BACKGROUND: The extracellular proteome or secretome of symbiotic bacteria like Rhizobium etli is presumed to be a key element of their infection strategy and survival. Rhizobia infect the roots of leguminous plants and establish a mutually beneficial symbiosis. To find out the possible role of secreted proteins we analyzed the extracellular proteome of R. etli CE3 in the exponential and stationary growth phases in minimal medium, supplemented with succinate-ammonium. RESULTS: The extracellular proteins were obtained by phenol extraction and identified by LC-ESI MS/MS. We identified 192 and 191 proteins for the exponential and stationary phases respectively. Using the software Signal P, we predicted signal peptides for 12.95% and 35.60% of the proteins identified in the exponential and stationary phases, respectively, which could therefore be secreted by the Sec pathway. For the exponential growth phase, we found in abundance proteins like the ribosomal proteins, toxins and proteins belonging to the group "defence mechanisms". For the stationary growth phase, we found that the most abundant proteins were those with unknown function, and in many of these we identified characteristic domains of proteases and peptidases. CONCLUSIONS: Our study provided the first dataset of the secretome of R. etli and its modifications, which may lead to novel insights into the adaptive response of different stages of growth. In addition, we found a high number of proteins with unknown function; these proteins could be analyzed in future research to elucidate their role in the extracellular proteome of R. etli. |
format | Text |
id | pubmed-2964644 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29646442010-10-28 The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase Meneses, Niurka Mendoza-Hernández, Guillermo Encarnación, Sergio Proteome Sci Research BACKGROUND: The extracellular proteome or secretome of symbiotic bacteria like Rhizobium etli is presumed to be a key element of their infection strategy and survival. Rhizobia infect the roots of leguminous plants and establish a mutually beneficial symbiosis. To find out the possible role of secreted proteins we analyzed the extracellular proteome of R. etli CE3 in the exponential and stationary growth phases in minimal medium, supplemented with succinate-ammonium. RESULTS: The extracellular proteins were obtained by phenol extraction and identified by LC-ESI MS/MS. We identified 192 and 191 proteins for the exponential and stationary phases respectively. Using the software Signal P, we predicted signal peptides for 12.95% and 35.60% of the proteins identified in the exponential and stationary phases, respectively, which could therefore be secreted by the Sec pathway. For the exponential growth phase, we found in abundance proteins like the ribosomal proteins, toxins and proteins belonging to the group "defence mechanisms". For the stationary growth phase, we found that the most abundant proteins were those with unknown function, and in many of these we identified characteristic domains of proteases and peptidases. CONCLUSIONS: Our study provided the first dataset of the secretome of R. etli and its modifications, which may lead to novel insights into the adaptive response of different stages of growth. In addition, we found a high number of proteins with unknown function; these proteins could be analyzed in future research to elucidate their role in the extracellular proteome of R. etli. BioMed Central 2010-10-14 /pmc/articles/PMC2964644/ /pubmed/20942974 http://dx.doi.org/10.1186/1477-5956-8-51 Text en Copyright ©2010 Meneses et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Meneses, Niurka Mendoza-Hernández, Guillermo Encarnación, Sergio The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase |
title | The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase |
title_full | The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase |
title_fullStr | The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase |
title_full_unstemmed | The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase |
title_short | The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase |
title_sort | extracellular proteome of rhizobium etli ce3 in exponential and stationary growth phase |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964644/ https://www.ncbi.nlm.nih.gov/pubmed/20942974 http://dx.doi.org/10.1186/1477-5956-8-51 |
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