Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method

BACKGROUND: The primer and amplicon length have been found to affect PCR based estimates of microbial diversity by pyrosequencing, while other PCR conditions have not been addressed using any deep sequencing method. The present study determined the effects of polymerase, template dilution and PCR cy...

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Detalles Bibliográficos
Autores principales: Wu, Jin-Ya, Jiang, Xiao-Tao, Jiang, Yun-Xia, Lu, Su-Ying, Zou, Fei, Zhou, Hong-Wei
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964677/
https://www.ncbi.nlm.nih.gov/pubmed/20937143
http://dx.doi.org/10.1186/1471-2180-10-255

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964677/
https://www.ncbi.nlm.nih.gov/pubmed/20937143
http://dx.doi.org/10.1186/1471-2180-10-255

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