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The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control

BACKGROUND: It is well established in E. coli and Vibrio cholerae that strains harboring mutations in the ferric uptake regulator gene (fur) are unable to utilize tricarboxylic acid (TCA) compounds, due to the down-regulation of key TCA cycle enzymes, such as AcnA and SdhABCD. This down-regulation i...

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Autores principales: Yang, Yunfeng, McCue, Lee Ann, Parsons, Andrea B, Feng, Sheng, Zhou, Jizhong
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964681/
https://www.ncbi.nlm.nih.gov/pubmed/20950482
http://dx.doi.org/10.1186/1471-2180-10-264
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author Yang, Yunfeng
McCue, Lee Ann
Parsons, Andrea B
Feng, Sheng
Zhou, Jizhong
author_facet Yang, Yunfeng
McCue, Lee Ann
Parsons, Andrea B
Feng, Sheng
Zhou, Jizhong
author_sort Yang, Yunfeng
collection PubMed
description BACKGROUND: It is well established in E. coli and Vibrio cholerae that strains harboring mutations in the ferric uptake regulator gene (fur) are unable to utilize tricarboxylic acid (TCA) compounds, due to the down-regulation of key TCA cycle enzymes, such as AcnA and SdhABCD. This down-regulation is mediated by a Fur-regulated small regulatory RNA named RyhB. It is unclear in the γ-proteobacterium S. oneidensis whether TCA is also regulated by Fur and RyhB. RESULTS: In the present study, we showed that a fur deletion mutant of S. oneidensis could utilize TCA compounds. Consistently, expression of the TCA cycle genes acnA and sdhA was not down-regulated in the mutant. To explore this observation further, we identified a ryhB gene in Shewanella species and experimentally demonstrated the gene expression. Further experiments suggested that RyhB was up-regulated in fur mutant, but that AcnA and SdhA were not controlled by RyhB. CONCLUSIONS: These cumulative results delineate an important difference of the Fur-RyhB regulatory cycle between S. oneidensis and other γ-proteobacteria. This work represents a step forward for understanding the unique regulation in S. oneidensis.
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spelling pubmed-29646812010-10-28 The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control Yang, Yunfeng McCue, Lee Ann Parsons, Andrea B Feng, Sheng Zhou, Jizhong BMC Microbiol Research Article BACKGROUND: It is well established in E. coli and Vibrio cholerae that strains harboring mutations in the ferric uptake regulator gene (fur) are unable to utilize tricarboxylic acid (TCA) compounds, due to the down-regulation of key TCA cycle enzymes, such as AcnA and SdhABCD. This down-regulation is mediated by a Fur-regulated small regulatory RNA named RyhB. It is unclear in the γ-proteobacterium S. oneidensis whether TCA is also regulated by Fur and RyhB. RESULTS: In the present study, we showed that a fur deletion mutant of S. oneidensis could utilize TCA compounds. Consistently, expression of the TCA cycle genes acnA and sdhA was not down-regulated in the mutant. To explore this observation further, we identified a ryhB gene in Shewanella species and experimentally demonstrated the gene expression. Further experiments suggested that RyhB was up-regulated in fur mutant, but that AcnA and SdhA were not controlled by RyhB. CONCLUSIONS: These cumulative results delineate an important difference of the Fur-RyhB regulatory cycle between S. oneidensis and other γ-proteobacteria. This work represents a step forward for understanding the unique regulation in S. oneidensis. BioMed Central 2010-10-16 /pmc/articles/PMC2964681/ /pubmed/20950482 http://dx.doi.org/10.1186/1471-2180-10-264 Text en Copyright ©2010 Yang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yang, Yunfeng
McCue, Lee Ann
Parsons, Andrea B
Feng, Sheng
Zhou, Jizhong
The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control
title The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control
title_full The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control
title_fullStr The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control
title_full_unstemmed The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control
title_short The tricarboxylic acid cycle in Shewanella oneidensis is independent of Fur and RyhB control
title_sort tricarboxylic acid cycle in shewanella oneidensis is independent of fur and ryhb control
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964681/
https://www.ncbi.nlm.nih.gov/pubmed/20950482
http://dx.doi.org/10.1186/1471-2180-10-264
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