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Binary IS Typing for Staphylococcus aureus

BACKGROUND: We present an easily applicable test for rapid binary typing of Staphylococcus aureus: binary interspace (IS) typing. This test is a further development of a previously described molecular typing technique that is based on length polymorphisms of the 16S-23S rDNA interspace region of S....

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Detalles Bibliográficos
Autores principales: Budding, Andries E., Vandenbroucke-Grauls, Christina M. J. E., Melles, Damian C., van Duijkeren, Engeline, Kluytmans, Jan A., Savelkoul, Paul H. M.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965118/
https://www.ncbi.nlm.nih.gov/pubmed/21060683
http://dx.doi.org/10.1371/journal.pone.0013671
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author Budding, Andries E.
Vandenbroucke-Grauls, Christina M. J. E.
Melles, Damian C.
van Duijkeren, Engeline
Kluytmans, Jan A.
Savelkoul, Paul H. M.
author_facet Budding, Andries E.
Vandenbroucke-Grauls, Christina M. J. E.
Melles, Damian C.
van Duijkeren, Engeline
Kluytmans, Jan A.
Savelkoul, Paul H. M.
author_sort Budding, Andries E.
collection PubMed
description BACKGROUND: We present an easily applicable test for rapid binary typing of Staphylococcus aureus: binary interspace (IS) typing. This test is a further development of a previously described molecular typing technique that is based on length polymorphisms of the 16S-23S rDNA interspace region of S. aureus. METHODOLOGY/PRINCIPAL FINDINGS: A novel approach of IS-typing was performed in which binary profiles are created. 424 human and animal derived MRSA and MSSA isolates were tested and a subset of these isolates was compared with multi locus sequence typing (MLST) and Amplified Fragment Length Polymorphism (AFLP). Binary IS typing had a high discriminatory potential and a good correlation with MLST and AFLP. CONCLUSIONS/SIGNIFICANCE: Binary IS typing is easy to perform and binary profiles can be generated in a standardized fashion. These two features, combined with the high correlation with MLST clonal complexes, make the technique applicable for large-scale inter-laboratory molecular epidemiological comparisons.
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spelling pubmed-29651182010-11-08 Binary IS Typing for Staphylococcus aureus Budding, Andries E. Vandenbroucke-Grauls, Christina M. J. E. Melles, Damian C. van Duijkeren, Engeline Kluytmans, Jan A. Savelkoul, Paul H. M. PLoS One Research Article BACKGROUND: We present an easily applicable test for rapid binary typing of Staphylococcus aureus: binary interspace (IS) typing. This test is a further development of a previously described molecular typing technique that is based on length polymorphisms of the 16S-23S rDNA interspace region of S. aureus. METHODOLOGY/PRINCIPAL FINDINGS: A novel approach of IS-typing was performed in which binary profiles are created. 424 human and animal derived MRSA and MSSA isolates were tested and a subset of these isolates was compared with multi locus sequence typing (MLST) and Amplified Fragment Length Polymorphism (AFLP). Binary IS typing had a high discriminatory potential and a good correlation with MLST and AFLP. CONCLUSIONS/SIGNIFICANCE: Binary IS typing is easy to perform and binary profiles can be generated in a standardized fashion. These two features, combined with the high correlation with MLST clonal complexes, make the technique applicable for large-scale inter-laboratory molecular epidemiological comparisons. Public Library of Science 2010-10-27 /pmc/articles/PMC2965118/ /pubmed/21060683 http://dx.doi.org/10.1371/journal.pone.0013671 Text en Budding et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Budding, Andries E.
Vandenbroucke-Grauls, Christina M. J. E.
Melles, Damian C.
van Duijkeren, Engeline
Kluytmans, Jan A.
Savelkoul, Paul H. M.
Binary IS Typing for Staphylococcus aureus
title Binary IS Typing for Staphylococcus aureus
title_full Binary IS Typing for Staphylococcus aureus
title_fullStr Binary IS Typing for Staphylococcus aureus
title_full_unstemmed Binary IS Typing for Staphylococcus aureus
title_short Binary IS Typing for Staphylococcus aureus
title_sort binary is typing for staphylococcus aureus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965118/
https://www.ncbi.nlm.nih.gov/pubmed/21060683
http://dx.doi.org/10.1371/journal.pone.0013671
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