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Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate
Two conserved catalytic arginines, Arg-173 and Arg-292, of the tyrosine site-specific recombinase Cre are essential for the transesterification steps of strand cleavage and joining in native DNA substrates containing scissile phosphate groups. The active site tyrosine (Tyr-324) provides the nucleoph...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965224/ https://www.ncbi.nlm.nih.gov/pubmed/20511594 http://dx.doi.org/10.1093/nar/gkq466 |