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High-quality gene assembly directly from unpurified mixtures of microarray-synthesized oligonucleotides
To meet the growing demand for synthetic genes more robust, scalable and inexpensive gene assembly technologies must be developed. Here, we present a protocol for high-quality gene assembly directly from low-cost marginal-quality microarray-synthesized oligonucleotides. Significantly, we eliminated...
| Autores principales: | , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965257/ https://www.ncbi.nlm.nih.gov/pubmed/20693531 http://dx.doi.org/10.1093/nar/gkq677 |