Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA

The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiol...

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Autores principales: Szwagierczak, Aleksandra, Bultmann, Sebastian, Schmidt, Christine S., Spada, Fabio, Leonhardt, Heinrich
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965258/
https://www.ncbi.nlm.nih.gov/pubmed/20685817
http://dx.doi.org/10.1093/nar/gkq684
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author Szwagierczak, Aleksandra
Bultmann, Sebastian
Schmidt, Christine S.
Spada, Fabio
Leonhardt, Heinrich
author_facet Szwagierczak, Aleksandra
Bultmann, Sebastian
Schmidt, Christine S.
Spada, Fabio
Leonhardt, Heinrich
author_sort Szwagierczak, Aleksandra
collection PubMed
description The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes.
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spelling pubmed-29652582010-10-28 Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA Szwagierczak, Aleksandra Bultmann, Sebastian Schmidt, Christine S. Spada, Fabio Leonhardt, Heinrich Nucleic Acids Res Methods Online The recent discovery of genomic 5-hydroxymethylcytosine (hmC) and mutations affecting the respective Tet hydroxylases in leukemia raises fundamental questions about this epigenetic modification. We present a sensitive method for fast quantification of genomic hmC based on specific transfer of radiolabeled glucose to hmC by a purified glucosyltransferase. We determined hmC levels in various adult tissues and differentiating embryonic stem cells and show a correlation with differential expression of tet genes. Oxford University Press 2010-10 2010-08-04 /pmc/articles/PMC2965258/ /pubmed/20685817 http://dx.doi.org/10.1093/nar/gkq684 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Online
Szwagierczak, Aleksandra
Bultmann, Sebastian
Schmidt, Christine S.
Spada, Fabio
Leonhardt, Heinrich
Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
title Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
title_full Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
title_fullStr Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
title_full_unstemmed Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
title_short Sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic DNA
title_sort sensitive enzymatic quantification of 5-hydroxymethylcytosine in genomic dna
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965258/
https://www.ncbi.nlm.nih.gov/pubmed/20685817
http://dx.doi.org/10.1093/nar/gkq684
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AT schmidtchristines sensitiveenzymaticquantificationof5hydroxymethylcytosineingenomicdna
AT spadafabio sensitiveenzymaticquantificationof5hydroxymethylcytosineingenomicdna
AT leonhardtheinrich sensitiveenzymaticquantificationof5hydroxymethylcytosineingenomicdna

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