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Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues

Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists...

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Autores principales: Barallon, Rita, Bauer, Steven R., Butler, John, Capes-Davis, Amanda, Dirks, Wilhelm G., Elmore, Eugene, Furtado, Manohar, Kline, Margaret C., Kohara, Arihiro, Los, Georgyi V., MacLeod, Roderick A. F., Masters, John R. W., Nardone, Mark, Nardone, Roland M., Nims, Raymond W., Price, Paul J., Reid, Yvonne A., Shewale, Jaiprakash, Sykes, Gregory, Steuer, Anton F., Storts, Douglas R., Thomson, Jim, Taraporewala, Zenobia, Alston-Roberts, Christine, Kerrigan, Liz
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965362/
https://www.ncbi.nlm.nih.gov/pubmed/20614197
http://dx.doi.org/10.1007/s11626-010-9333-z
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author Barallon, Rita
Bauer, Steven R.
Butler, John
Capes-Davis, Amanda
Dirks, Wilhelm G.
Elmore, Eugene
Furtado, Manohar
Kline, Margaret C.
Kohara, Arihiro
Los, Georgyi V.
MacLeod, Roderick A. F.
Masters, John R. W.
Nardone, Mark
Nardone, Roland M.
Nims, Raymond W.
Price, Paul J.
Reid, Yvonne A.
Shewale, Jaiprakash
Sykes, Gregory
Steuer, Anton F.
Storts, Douglas R.
Thomson, Jim
Taraporewala, Zenobia
Alston-Roberts, Christine
Kerrigan, Liz
author_facet Barallon, Rita
Bauer, Steven R.
Butler, John
Capes-Davis, Amanda
Dirks, Wilhelm G.
Elmore, Eugene
Furtado, Manohar
Kline, Margaret C.
Kohara, Arihiro
Los, Georgyi V.
MacLeod, Roderick A. F.
Masters, John R. W.
Nardone, Mark
Nardone, Roland M.
Nims, Raymond W.
Price, Paul J.
Reid, Yvonne A.
Shewale, Jaiprakash
Sykes, Gregory
Steuer, Anton F.
Storts, Douglas R.
Thomson, Jim
Taraporewala, Zenobia
Alston-Roberts, Christine
Kerrigan, Liz
author_sort Barallon, Rita
collection PubMed
description Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer review of papers submitted for publication or during review of grants submitted for funding. In order to facilitate proper identity testing, accurate, reliable, inexpensive, and standardized methods for authentication of cells and cell lines must be made available. To this end, an international team of scientists is, at this time, preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. This standard, which will be submitted for review and approval as an American National Standard by the American National Standards Institute, will provide investigators guidance on the use of STR profiling for authenticating human cell lines. Such guidance will include methodological detail on the preparation of the DNA sample, the appropriate numbers and types of loci to be evaluated, and the interpretation and quality control of the results. Associated with the standard itself will be the establishment and maintenance of a public STR profile database under the auspices of the National Center for Biotechnology Information. The consensus standard is anticipated to be adopted by granting agencies and scientific journals as appropriate methodology for authenticating human cell lines, stem cells, and tissues.
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spelling pubmed-29653622010-11-16 Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues Barallon, Rita Bauer, Steven R. Butler, John Capes-Davis, Amanda Dirks, Wilhelm G. Elmore, Eugene Furtado, Manohar Kline, Margaret C. Kohara, Arihiro Los, Georgyi V. MacLeod, Roderick A. F. Masters, John R. W. Nardone, Mark Nardone, Roland M. Nims, Raymond W. Price, Paul J. Reid, Yvonne A. Shewale, Jaiprakash Sykes, Gregory Steuer, Anton F. Storts, Douglas R. Thomson, Jim Taraporewala, Zenobia Alston-Roberts, Christine Kerrigan, Liz In Vitro Cell Dev Biol Anim Invited Review Cell misidentification and cross-contamination have plagued biomedical research for as long as cells have been employed as research tools. Examples of misidentified cell lines continue to surface to this day. Efforts to eradicate the problem by raising awareness of the issue and by asking scientists voluntarily to take appropriate actions have not been successful. Unambiguous cell authentication is an essential step in the scientific process and should be an inherent consideration during peer review of papers submitted for publication or during review of grants submitted for funding. In order to facilitate proper identity testing, accurate, reliable, inexpensive, and standardized methods for authentication of cells and cell lines must be made available. To this end, an international team of scientists is, at this time, preparing a consensus standard on the authentication of human cells using short tandem repeat (STR) profiling. This standard, which will be submitted for review and approval as an American National Standard by the American National Standards Institute, will provide investigators guidance on the use of STR profiling for authenticating human cell lines. Such guidance will include methodological detail on the preparation of the DNA sample, the appropriate numbers and types of loci to be evaluated, and the interpretation and quality control of the results. Associated with the standard itself will be the establishment and maintenance of a public STR profile database under the auspices of the National Center for Biotechnology Information. The consensus standard is anticipated to be adopted by granting agencies and scientific journals as appropriate methodology for authenticating human cell lines, stem cells, and tissues. Springer-Verlag 2010-07-08 2010 /pmc/articles/PMC2965362/ /pubmed/20614197 http://dx.doi.org/10.1007/s11626-010-9333-z Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Invited Review
Barallon, Rita
Bauer, Steven R.
Butler, John
Capes-Davis, Amanda
Dirks, Wilhelm G.
Elmore, Eugene
Furtado, Manohar
Kline, Margaret C.
Kohara, Arihiro
Los, Georgyi V.
MacLeod, Roderick A. F.
Masters, John R. W.
Nardone, Mark
Nardone, Roland M.
Nims, Raymond W.
Price, Paul J.
Reid, Yvonne A.
Shewale, Jaiprakash
Sykes, Gregory
Steuer, Anton F.
Storts, Douglas R.
Thomson, Jim
Taraporewala, Zenobia
Alston-Roberts, Christine
Kerrigan, Liz
Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues
title Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues
title_full Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues
title_fullStr Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues
title_full_unstemmed Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues
title_short Recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues
title_sort recommendation of short tandem repeat profiling for authenticating human cell lines, stem cells, and tissues
topic Invited Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965362/
https://www.ncbi.nlm.nih.gov/pubmed/20614197
http://dx.doi.org/10.1007/s11626-010-9333-z
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