Cargando…

The transcriptional response of Pasteurella multocida to three classes of antibiotics

BACKGROUND: Pasteurella multocida is a gram-negative bacterial pathogen that has a broad host range. One of the diseases it causes is fowl cholera in poultry. The availability of the genome sequence of avian P. multocida isolate Pm70 enables the application of functional genomics for observing globa...

Descripción completa

Detalles Bibliográficos
Autores principales: Nanduri, Bindu, Shack, Leslie A, Burgess, Shane C, Lawrence, Mark L
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2966327/
https://www.ncbi.nlm.nih.gov/pubmed/19607655
http://dx.doi.org/10.1186/1471-2164-10-S2-S4
_version_ 1782189568790888448
author Nanduri, Bindu
Shack, Leslie A
Burgess, Shane C
Lawrence, Mark L
author_facet Nanduri, Bindu
Shack, Leslie A
Burgess, Shane C
Lawrence, Mark L
author_sort Nanduri, Bindu
collection PubMed
description BACKGROUND: Pasteurella multocida is a gram-negative bacterial pathogen that has a broad host range. One of the diseases it causes is fowl cholera in poultry. The availability of the genome sequence of avian P. multocida isolate Pm70 enables the application of functional genomics for observing global gene expression in response to a given stimulus. We studied the effects of three classes of antibiotics on the P. multocida transcriptome using custom oligonucleotide microarrays from NimbleGen Systems. Hybridizations were conducted with RNA isolated from three independent cultures of Pm70 grown in the presence or absence of sub-minimum inhibitory concentration (sub-MIC) of antibiotics. Differentially expressed (DE) genes were identified by ANOVA and Dunnett's test. Biological modeling of the differentially expressed genes (DE) was conducted based on Clusters of Orthologous (COG) groups and network analysis in Pathway Studio. RESULTS: The three antibiotics used in this study, amoxicillin, chlortetracycline, and enrofloxacin, collectively influenced transcription of 25% of the P. multocida Pm70 genome. Some DE genes identified were common to more than one antibiotic. The overall transcription signatures of the three antibiotics differed at the COG level of the analysis. Network analysis identified differences in the SOS response of P. multocida in response to the antibiotics. CONCLUSION: This is the first report of the transcriptional response of an avian strain of P. multocida to sub-lethal concentrations of three different classes of antibiotics. We identified common adaptive responses of P. multocida to antibiotic stress. The observed changes in gene expression of known and putative P. multocida virulence factors establish the molecular basis for the therapeutic efficacy of sub-MICs. Our network analysis demonstrates the feasibility and limitations of applying systems modeling to high throughput datasets in 'non-model' bacteria.
format Text
id pubmed-2966327
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-29663272010-10-29 The transcriptional response of Pasteurella multocida to three classes of antibiotics Nanduri, Bindu Shack, Leslie A Burgess, Shane C Lawrence, Mark L BMC Genomics Proceedings BACKGROUND: Pasteurella multocida is a gram-negative bacterial pathogen that has a broad host range. One of the diseases it causes is fowl cholera in poultry. The availability of the genome sequence of avian P. multocida isolate Pm70 enables the application of functional genomics for observing global gene expression in response to a given stimulus. We studied the effects of three classes of antibiotics on the P. multocida transcriptome using custom oligonucleotide microarrays from NimbleGen Systems. Hybridizations were conducted with RNA isolated from three independent cultures of Pm70 grown in the presence or absence of sub-minimum inhibitory concentration (sub-MIC) of antibiotics. Differentially expressed (DE) genes were identified by ANOVA and Dunnett's test. Biological modeling of the differentially expressed genes (DE) was conducted based on Clusters of Orthologous (COG) groups and network analysis in Pathway Studio. RESULTS: The three antibiotics used in this study, amoxicillin, chlortetracycline, and enrofloxacin, collectively influenced transcription of 25% of the P. multocida Pm70 genome. Some DE genes identified were common to more than one antibiotic. The overall transcription signatures of the three antibiotics differed at the COG level of the analysis. Network analysis identified differences in the SOS response of P. multocida in response to the antibiotics. CONCLUSION: This is the first report of the transcriptional response of an avian strain of P. multocida to sub-lethal concentrations of three different classes of antibiotics. We identified common adaptive responses of P. multocida to antibiotic stress. The observed changes in gene expression of known and putative P. multocida virulence factors establish the molecular basis for the therapeutic efficacy of sub-MICs. Our network analysis demonstrates the feasibility and limitations of applying systems modeling to high throughput datasets in 'non-model' bacteria. BioMed Central 2009-07-14 /pmc/articles/PMC2966327/ /pubmed/19607655 http://dx.doi.org/10.1186/1471-2164-10-S2-S4 Text en Copyright ©2009 Nanduri et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Proceedings
Nanduri, Bindu
Shack, Leslie A
Burgess, Shane C
Lawrence, Mark L
The transcriptional response of Pasteurella multocida to three classes of antibiotics
title The transcriptional response of Pasteurella multocida to three classes of antibiotics
title_full The transcriptional response of Pasteurella multocida to three classes of antibiotics
title_fullStr The transcriptional response of Pasteurella multocida to three classes of antibiotics
title_full_unstemmed The transcriptional response of Pasteurella multocida to three classes of antibiotics
title_short The transcriptional response of Pasteurella multocida to three classes of antibiotics
title_sort transcriptional response of pasteurella multocida to three classes of antibiotics
topic Proceedings
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2966327/
https://www.ncbi.nlm.nih.gov/pubmed/19607655
http://dx.doi.org/10.1186/1471-2164-10-S2-S4
work_keys_str_mv AT nanduribindu thetranscriptionalresponseofpasteurellamultocidatothreeclassesofantibiotics
AT shacklesliea thetranscriptionalresponseofpasteurellamultocidatothreeclassesofantibiotics
AT burgessshanec thetranscriptionalresponseofpasteurellamultocidatothreeclassesofantibiotics
AT lawrencemarkl thetranscriptionalresponseofpasteurellamultocidatothreeclassesofantibiotics
AT nanduribindu transcriptionalresponseofpasteurellamultocidatothreeclassesofantibiotics
AT shacklesliea transcriptionalresponseofpasteurellamultocidatothreeclassesofantibiotics
AT burgessshanec transcriptionalresponseofpasteurellamultocidatothreeclassesofantibiotics
AT lawrencemarkl transcriptionalresponseofpasteurellamultocidatothreeclassesofantibiotics