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Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain

Pre-clinical bone cancer pain models mimicking the human condition are required to respond to clinical realities. Breast or prostate cancer patients coping with bone metastases experience intractable pain, which affects their quality of life. Advanced monitoring is thus required to clarify bone canc...

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Autores principales: Doré-Savard, Louis, Otis, Valérie, Belleville, Karine, Lemire, Myriam, Archambault, Mélanie, Tremblay, Luc, Beaudoin, Jean-François, Beaudet, Nicolas, Lecomte, Roger, Lepage, Martin, Gendron, Louis, Sarret, Philippe
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2966439/
https://www.ncbi.nlm.nih.gov/pubmed/21048940
http://dx.doi.org/10.1371/journal.pone.0013774
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author Doré-Savard, Louis
Otis, Valérie
Belleville, Karine
Lemire, Myriam
Archambault, Mélanie
Tremblay, Luc
Beaudoin, Jean-François
Beaudet, Nicolas
Lecomte, Roger
Lepage, Martin
Gendron, Louis
Sarret, Philippe
author_facet Doré-Savard, Louis
Otis, Valérie
Belleville, Karine
Lemire, Myriam
Archambault, Mélanie
Tremblay, Luc
Beaudoin, Jean-François
Beaudet, Nicolas
Lecomte, Roger
Lepage, Martin
Gendron, Louis
Sarret, Philippe
author_sort Doré-Savard, Louis
collection PubMed
description Pre-clinical bone cancer pain models mimicking the human condition are required to respond to clinical realities. Breast or prostate cancer patients coping with bone metastases experience intractable pain, which affects their quality of life. Advanced monitoring is thus required to clarify bone cancer pain mechanisms and refine treatments. In our model of rat femoral mammary carcinoma MRMT-1 cell implantation, pain onset and tumor growth were monitored for 21 days. The surgical procedure performed without arthrotomy allowed recording of incidental pain in free-moving rats. Along with the gradual development of mechanical allodynia and hyperalgesia, behavioral signs of ambulatory pain were detected at day 14 by using a dynamic weight-bearing apparatus. Osteopenia was revealed from day 14 concomitantly with disorganization of the trabecular architecture (µCT). Bone metastases were visualized as early as day 8 by MRI (T(1)-Gd-DTPA) before pain detection. PET (Na(18)F) co-registration revealed intra-osseous activity, as determined by anatomical superimposition over MRI in accordance with osteoclastic hyperactivity (TRAP staining). Pain and bone destruction were aggravated with time. Bone remodeling was accompanied by c-Fos (spinal) and ATF3 (DRG) neuronal activation, sustained by astrocyte (GFAP) and microglia (Iba1) reactivity in lumbar spinal cord. Our animal model demonstrates the importance of simultaneously recording pain and tumor progression and will allow us to better characterize therapeutic strategies in the future.
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spelling pubmed-29664392010-11-03 Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain Doré-Savard, Louis Otis, Valérie Belleville, Karine Lemire, Myriam Archambault, Mélanie Tremblay, Luc Beaudoin, Jean-François Beaudet, Nicolas Lecomte, Roger Lepage, Martin Gendron, Louis Sarret, Philippe PLoS One Research Article Pre-clinical bone cancer pain models mimicking the human condition are required to respond to clinical realities. Breast or prostate cancer patients coping with bone metastases experience intractable pain, which affects their quality of life. Advanced monitoring is thus required to clarify bone cancer pain mechanisms and refine treatments. In our model of rat femoral mammary carcinoma MRMT-1 cell implantation, pain onset and tumor growth were monitored for 21 days. The surgical procedure performed without arthrotomy allowed recording of incidental pain in free-moving rats. Along with the gradual development of mechanical allodynia and hyperalgesia, behavioral signs of ambulatory pain were detected at day 14 by using a dynamic weight-bearing apparatus. Osteopenia was revealed from day 14 concomitantly with disorganization of the trabecular architecture (µCT). Bone metastases were visualized as early as day 8 by MRI (T(1)-Gd-DTPA) before pain detection. PET (Na(18)F) co-registration revealed intra-osseous activity, as determined by anatomical superimposition over MRI in accordance with osteoclastic hyperactivity (TRAP staining). Pain and bone destruction were aggravated with time. Bone remodeling was accompanied by c-Fos (spinal) and ATF3 (DRG) neuronal activation, sustained by astrocyte (GFAP) and microglia (Iba1) reactivity in lumbar spinal cord. Our animal model demonstrates the importance of simultaneously recording pain and tumor progression and will allow us to better characterize therapeutic strategies in the future. Public Library of Science 2010-10-29 /pmc/articles/PMC2966439/ /pubmed/21048940 http://dx.doi.org/10.1371/journal.pone.0013774 Text en Doré-Savard et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Doré-Savard, Louis
Otis, Valérie
Belleville, Karine
Lemire, Myriam
Archambault, Mélanie
Tremblay, Luc
Beaudoin, Jean-François
Beaudet, Nicolas
Lecomte, Roger
Lepage, Martin
Gendron, Louis
Sarret, Philippe
Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain
title Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain
title_full Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain
title_fullStr Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain
title_full_unstemmed Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain
title_short Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain
title_sort behavioral, medical imaging and histopathological features of a new rat model of bone cancer pain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2966439/
https://www.ncbi.nlm.nih.gov/pubmed/21048940
http://dx.doi.org/10.1371/journal.pone.0013774
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