Cargando…

Enumeration of islets by nuclei counting and light microscopic analysis

Islet enumeration in impure preparations by conventional dithizone staining and visual counting is inaccurate and operator dependent. We examined nuclei counting for measuring the total number of cells in islet preparations, and we combined it with morphological analysis by light microscopy (LM) for...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pisania, Anna, Papas, Klearchos K., Powers, Daryl E., Rappel, Michael J., Omer, Abdulkadir, Bonner-Weir, Susan, Weir, Gordon C., Colton, Clark K.
Formato:	Texto
Lenguaje:	English
Publicado:	2010
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2966546/ https://www.ncbi.nlm.nih.gov/pubmed/20697375 http://dx.doi.org/10.1038/labinvest.2010.125

Ejemplares similares

Quantitative analysis of cell composition and purity of human pancreatic islet preparations
por: Pisania, Anna, et al.
Publicado: (2010)

Sleeping Islets and the Relationship Between β-Cell Mass and Function
por: Weir, Gordon C., et al.
Publicado: (2011)

Oxygenation of the Intraportally Transplanted Pancreatic Islet
por: Suszynski, Thomas M., et al.
Publicado: (2016)

Islet β cell mass in diabetes and how it relates to function, birth, and death
por: Weir, Gordon C, et al.
Publicado: (2013)

Islet Oxygen Consumption Rate (OCR) Dose Predicts Insulin Independence in Clinical Islet Autotransplantation
por: Papas, Klearchos K., et al.
Publicado: (2015)

Why pancreatic islets should be regarded and regulated like organs
por: Weir, G. C., et al.
Publicado: (2021)

Regulation of the JNK3 Signaling Pathway during Islet Isolation: JNK3 and c-fos as New Markers of Islet Quality for Transplantation
por: Abdelli, Saida, et al.
Publicado: (2014)

Islets in Type 2 Diabetes: In Honor of Dr. Robert C. Turner
por: Bonner-Weir, Susan, et al.
Publicado: (2008)

Single pancreatic beta cells co-express multiple islet hormone genes in mice
por: Katsuta, H., et al.
Publicado: (2009)

Glucose Driven Changes in Beta Cell Identity Are Important for Function and Possibly Autoimmune Vulnerability during the Progression of Type 1 Diabetes
por: Weir, Gordon C., et al.
Publicado: (2017)

Induction of remission in diabetes by lowering blood glucose
por: Weir, Gordon C., et al.
Publicado: (2023)

Conflicting Views About Interactions Between Pancreatic α-Cells and β-Cells
por: Weir, Gordon C., et al.
Publicado: (2023)

Enumerating β-Cells in Whole Human Islets: Sex Differences and Associations With Clinical Outcomes After Islet Transplantation
por: Marchese, Enza, et al.
Publicado: (2015)

Islet Harvest in Carbon Monoxide-Saturated Medium for Chronic Pancreatitis Patients Undergoing Islet Autotransplantation
por: Wang, Hongjun, et al.
Publicado: (2019)

Sustained NF-κB Activation and Inhibition in β-Cells Have Minimal Effects on Function and Islet Transplant Outcomes
por: King, Aileen J. F., et al.
Publicado: (2013)

Continuous Quadrupole Magnetic Separation of Islets during Digestion Improves Purified Porcine Islet Viability
por: Weegman, Bradley P., et al.
Publicado: (2016)

Stem cell approaches for diabetes: towards beta cell replacement
por: Weir, Gordon C, et al.
Publicado: (2011)

Dynamic development of the pancreas from birth to adulthood
por: Bonner-Weir, Susan, et al.
Publicado: (2016)

β-cell dedifferentiation in diabetes is important, but what is it?
por: Weir, Gordon C, et al.
Publicado: (2013)

Method validation of circulating tumour cell enumeration at low cell counts
por: Cummings, Jeffrey, et al.
Publicado: (2013)

(p,q)-biclique counting and enumeration for large sparse bipartite graphs
por: Yang, Jianye, et al.
Publicado: (2023)

SUN-LB059 A Rare Case Of Hypoglycemia Secondary To Proinsulin-Intermediate Secreting Tumor
por: Zainal, Abir, et al.
Publicado: (2019)

The Efficacy of an Immunoisolating Membrane System for Islet Xenotransplantation in Minipigs
por: Neufeld, Tova, et al.
Publicado: (2013)

Preliminary Studies of the Impact of CXCL12 on the Foreign Body Reaction to Pancreatic Islets Microencapsulated in Alginate in Nonhuman Primates
por: Sremac, Marinko, et al.
Publicado: (2019)

Reduced Ki67 Staining in the Postmortem State Calls Into Question Past Conclusions About the Lack of Turnover of Adult Human β-Cells
por: Sullivan, Brooke A., et al.
Publicado: (2015)

An aptasensor based on the microscopic enumeration of encoding gold nanoparticles for the detection of C-reactive protein
por: Zhao, Yuanfang, et al.
Publicado: (2019)

Strategies for enumeration of circulating microvesicles on a conventional flow cytometer: Counting beads and scatter parameters
por: Alkhatatbeh, Mohammad J, et al.
Publicado: (2018)

Encapsulated Cells for the Treatment of Diabetes: Danger of Acute Hypoglycemia Following Injury?
por: Papas, Klearchos K., et al.
Publicado: (2023)

Microscopic approach to the scattering of unstable nuclei at intermediate incident energies
por: Yahiro, M, et al.
Publicado: (2012)

STUDIES ON NUCLEI USING CORRELATED CYTOCHEMICAL, LIGHT, AND ELECTRON MICROSCOPE TECHNIQUES
por: Moses, Montrose J.
Publicado: (1956)

International Symposium on Microscopic Cluster Models of Light Nuclei and Related Topics
Publicado: (1992)

Postnatal β2 adrenergic treatment improves insulin sensitivity in lambs with IUGR but not persistent defects in pancreatic islets or skeletal muscle
por: Yates, Dustin T., et al.
Publicado: (2019)

Enumerating Sparse Organisms in Ships’ Ballast Water: Why Counting to 10 Is Not So Easy
por: Miller, A. Whitman, et al.
Publicado: (2011)

Interferon-gamma drives programmed death-ligand 1 expression on islet β cells to limit T cell function during autoimmune diabetes
por: Osum, Kevin C., et al.
Publicado: (2018)

Long-term viability and function of transplanted islets macroencapsulated at high density are achieved by enhanced oxygen supply
por: Evron, Yoav, et al.
Publicado: (2018)

A review of microscopic cell imaging and neural network recognition for synergistic cyanobacteria identification and enumeration
por: Vaughan, Liam, et al.
Publicado: (2022)

Realistic microscopic level densities for spherical nuclei
por: Cerf, N J
Publicado: (1994)

Microscopic description of the radiative pion capture in nuclei
por: Delorme, J, et al.
Publicado: (1974)

β-Cell Growth and Regeneration: Replication Is Only Part of the Story
por: Bonner-Weir, Susan, et al.
Publicado: (2010)

Graphical enumeration
por: Harary, Frank, et al.
Publicado: (1973)

Cannot write session to /tmp/vufind_sessions/sess_saldrigp04ofti5c0a1b6al75h