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Study of the Molecular Recognition of Aptamers Selected through Ovarian Cancer Cell-SELEX

BACKGROUND: Ovarian cancer is the most lethal gynecological malignancy, and the ovarian clear cell carcinoma subtype (OCCA) demonstrates a particularly poor response to standard treatment. Improvements in ovarian cancer outcomes, especially for OCCA, could be expected from a clearer understanding of...

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Autores principales: Van Simaeys, Dimitri, López-Colón, Dalia, Sefah, Kwame, Sutphen, Rebecca, Jimenez, Elizabeth, Tan, Weihong
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2967474/
https://www.ncbi.nlm.nih.gov/pubmed/21072169
http://dx.doi.org/10.1371/journal.pone.0013770
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author Van Simaeys, Dimitri
López-Colón, Dalia
Sefah, Kwame
Sutphen, Rebecca
Jimenez, Elizabeth
Tan, Weihong
author_facet Van Simaeys, Dimitri
López-Colón, Dalia
Sefah, Kwame
Sutphen, Rebecca
Jimenez, Elizabeth
Tan, Weihong
author_sort Van Simaeys, Dimitri
collection PubMed
description BACKGROUND: Ovarian cancer is the most lethal gynecological malignancy, and the ovarian clear cell carcinoma subtype (OCCA) demonstrates a particularly poor response to standard treatment. Improvements in ovarian cancer outcomes, especially for OCCA, could be expected from a clearer understanding of the molecular pathology that might guide strategies for earlier diagnosis and more effective treatment. METHODOLOGY/PRINCIPAL FINDINGS: Cell-SELEX technology was employed to develop new molecular probes for ovarian cancer cell surface markers. A total of thirteen aptamers with K(d)'s to ovarian cancer cells in the pico- to nanomolar range were obtained. Preliminary investigation of the targets of these aptamers and their binding characteristics was also performed. CONCLUSIONS/SIGNIFICANCE: We have selected a series of aptamers that bind to different types of ovarian cancer, but not cervical cancer. Though binding to other cancer cell lines was observed, these aptamers could lead to identification of biomarkers that are related to cancer.
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spelling pubmed-29674742010-11-10 Study of the Molecular Recognition of Aptamers Selected through Ovarian Cancer Cell-SELEX Van Simaeys, Dimitri López-Colón, Dalia Sefah, Kwame Sutphen, Rebecca Jimenez, Elizabeth Tan, Weihong PLoS One Research Article BACKGROUND: Ovarian cancer is the most lethal gynecological malignancy, and the ovarian clear cell carcinoma subtype (OCCA) demonstrates a particularly poor response to standard treatment. Improvements in ovarian cancer outcomes, especially for OCCA, could be expected from a clearer understanding of the molecular pathology that might guide strategies for earlier diagnosis and more effective treatment. METHODOLOGY/PRINCIPAL FINDINGS: Cell-SELEX technology was employed to develop new molecular probes for ovarian cancer cell surface markers. A total of thirteen aptamers with K(d)'s to ovarian cancer cells in the pico- to nanomolar range were obtained. Preliminary investigation of the targets of these aptamers and their binding characteristics was also performed. CONCLUSIONS/SIGNIFICANCE: We have selected a series of aptamers that bind to different types of ovarian cancer, but not cervical cancer. Though binding to other cancer cell lines was observed, these aptamers could lead to identification of biomarkers that are related to cancer. Public Library of Science 2010-11-01 /pmc/articles/PMC2967474/ /pubmed/21072169 http://dx.doi.org/10.1371/journal.pone.0013770 Text en Van Simaeys et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Van Simaeys, Dimitri
López-Colón, Dalia
Sefah, Kwame
Sutphen, Rebecca
Jimenez, Elizabeth
Tan, Weihong
Study of the Molecular Recognition of Aptamers Selected through Ovarian Cancer Cell-SELEX
title Study of the Molecular Recognition of Aptamers Selected through Ovarian Cancer Cell-SELEX
title_full Study of the Molecular Recognition of Aptamers Selected through Ovarian Cancer Cell-SELEX
title_fullStr Study of the Molecular Recognition of Aptamers Selected through Ovarian Cancer Cell-SELEX
title_full_unstemmed Study of the Molecular Recognition of Aptamers Selected through Ovarian Cancer Cell-SELEX
title_short Study of the Molecular Recognition of Aptamers Selected through Ovarian Cancer Cell-SELEX
title_sort study of the molecular recognition of aptamers selected through ovarian cancer cell-selex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2967474/
https://www.ncbi.nlm.nih.gov/pubmed/21072169
http://dx.doi.org/10.1371/journal.pone.0013770
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