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Maternal Rnf12/RLIM is required for imprinted X chromosome inactivation in mice

Two forms of XCI ensure the selective silencing of female sex chromosomes during mouse embryogenesis. Imprinted XCI begins with the detection of Xist RNA expression on the paternal X chromosome (Xp) around the four cell stage of embryonic development. In the embryonic tissues of the inner cell mass...

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Autores principales: Shin, JongDae, Bossenz, Michael, Chung, Young, Ma, Hong, Byron, Meg, Taniguchi-Ishigaki, Naoko, Zhu, Xiaochun, Jiao, Baowei, Hall, Lisa L., Green, Michael R., Jones, Stephen N., Hermans-Borgmeyer, Irm, Lawrence, Jeanne B., Bach, Ingolf
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2967734/
https://www.ncbi.nlm.nih.gov/pubmed/20962847
http://dx.doi.org/10.1038/nature09457
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author Shin, JongDae
Bossenz, Michael
Chung, Young
Ma, Hong
Byron, Meg
Taniguchi-Ishigaki, Naoko
Zhu, Xiaochun
Jiao, Baowei
Hall, Lisa L.
Green, Michael R.
Jones, Stephen N.
Hermans-Borgmeyer, Irm
Lawrence, Jeanne B.
Bach, Ingolf
author_facet Shin, JongDae
Bossenz, Michael
Chung, Young
Ma, Hong
Byron, Meg
Taniguchi-Ishigaki, Naoko
Zhu, Xiaochun
Jiao, Baowei
Hall, Lisa L.
Green, Michael R.
Jones, Stephen N.
Hermans-Borgmeyer, Irm
Lawrence, Jeanne B.
Bach, Ingolf
author_sort Shin, JongDae
collection PubMed
description Two forms of XCI ensure the selective silencing of female sex chromosomes during mouse embryogenesis. Imprinted XCI begins with the detection of Xist RNA expression on the paternal X chromosome (Xp) around the four cell stage of embryonic development. In the embryonic tissues of the inner cell mass (ICM), a random form of XCI occurs in blastocysts which inactivates either the Xp or the maternal X chromosome (Xm) 1,2. Both forms of XCI require the non-coding Xist RNA which coats the inactive X chromosome (Xi) from which it is expressed. Xist plays crucial functions for the silencing of X-linked genes including Rnf12 3,4 encoding the ubiquitin ligase RLIM. Targeting a conditional knockout (KO) of Rnf12 to oocytes where RLIM accumulates to high levels, we find that the maternal transmission of the mutant X chromosome (Δm) leads to embryonic lethality due to defective imprinted XCI. We show that in Δm female embryos the initial formation of Xist clouds and Xp silencing is inhibited. In contrast, ES cells lacking RLIM are able to form Xist clouds and silence at least some X-linked genes during random XCI. These results assign crucial roles to the maternal deposit of Rnf12/RLIM for the initiation of imprinted XCI.
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spelling pubmed-29677342011-04-21 Maternal Rnf12/RLIM is required for imprinted X chromosome inactivation in mice Shin, JongDae Bossenz, Michael Chung, Young Ma, Hong Byron, Meg Taniguchi-Ishigaki, Naoko Zhu, Xiaochun Jiao, Baowei Hall, Lisa L. Green, Michael R. Jones, Stephen N. Hermans-Borgmeyer, Irm Lawrence, Jeanne B. Bach, Ingolf Nature Article Two forms of XCI ensure the selective silencing of female sex chromosomes during mouse embryogenesis. Imprinted XCI begins with the detection of Xist RNA expression on the paternal X chromosome (Xp) around the four cell stage of embryonic development. In the embryonic tissues of the inner cell mass (ICM), a random form of XCI occurs in blastocysts which inactivates either the Xp or the maternal X chromosome (Xm) 1,2. Both forms of XCI require the non-coding Xist RNA which coats the inactive X chromosome (Xi) from which it is expressed. Xist plays crucial functions for the silencing of X-linked genes including Rnf12 3,4 encoding the ubiquitin ligase RLIM. Targeting a conditional knockout (KO) of Rnf12 to oocytes where RLIM accumulates to high levels, we find that the maternal transmission of the mutant X chromosome (Δm) leads to embryonic lethality due to defective imprinted XCI. We show that in Δm female embryos the initial formation of Xist clouds and Xp silencing is inhibited. In contrast, ES cells lacking RLIM are able to form Xist clouds and silence at least some X-linked genes during random XCI. These results assign crucial roles to the maternal deposit of Rnf12/RLIM for the initiation of imprinted XCI. 2010-10-21 /pmc/articles/PMC2967734/ /pubmed/20962847 http://dx.doi.org/10.1038/nature09457 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Shin, JongDae
Bossenz, Michael
Chung, Young
Ma, Hong
Byron, Meg
Taniguchi-Ishigaki, Naoko
Zhu, Xiaochun
Jiao, Baowei
Hall, Lisa L.
Green, Michael R.
Jones, Stephen N.
Hermans-Borgmeyer, Irm
Lawrence, Jeanne B.
Bach, Ingolf
Maternal Rnf12/RLIM is required for imprinted X chromosome inactivation in mice
title Maternal Rnf12/RLIM is required for imprinted X chromosome inactivation in mice
title_full Maternal Rnf12/RLIM is required for imprinted X chromosome inactivation in mice
title_fullStr Maternal Rnf12/RLIM is required for imprinted X chromosome inactivation in mice
title_full_unstemmed Maternal Rnf12/RLIM is required for imprinted X chromosome inactivation in mice
title_short Maternal Rnf12/RLIM is required for imprinted X chromosome inactivation in mice
title_sort maternal rnf12/rlim is required for imprinted x chromosome inactivation in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2967734/
https://www.ncbi.nlm.nih.gov/pubmed/20962847
http://dx.doi.org/10.1038/nature09457
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