Cargando…
Localization and Androgen Regulation of Metastasis-Associated Protein 1 in Mouse Epididymis
BACKGROUND: Metastasis-associated protein 1 (MTA1), the founding member of the MTA family of genes, can modulate transcription by influencing the status of chromatin remodeling. Despite its strong correlation with the metastatic potential of cancer cells, MTA1 can also regulate crucial cellular path...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2972736/ https://www.ncbi.nlm.nih.gov/pubmed/21082030 http://dx.doi.org/10.1371/journal.pone.0015439 |
_version_ | 1782190827282366464 |
---|---|
author | Ma, Li Li, Wei Zhu, Hua-Ping Li, Zhen Sun, Zhi-Jian Liu, Xin-Ping Zhao, Jie Zhang, Jin-Shan Zhang, Yuan-Qiang |
author_facet | Ma, Li Li, Wei Zhu, Hua-Ping Li, Zhen Sun, Zhi-Jian Liu, Xin-Ping Zhao, Jie Zhang, Jin-Shan Zhang, Yuan-Qiang |
author_sort | Ma, Li |
collection | PubMed |
description | BACKGROUND: Metastasis-associated protein 1 (MTA1), the founding member of the MTA family of genes, can modulate transcription by influencing the status of chromatin remodeling. Despite its strong correlation with the metastatic potential of cancer cells, MTA1 can also regulate crucial cellular pathways by modifying the acetylation status. We have previously reported the presence of MTA1/MTA1 in human and mouse testes, providing the evidence for its involvement in the regulation of testicular function during murine spermatogenesis. The objective of present study was to further assess the localization of MTA1 in mouse epididymis on both transcriptional and translational level, and then to explore whether MTA1 expression is regulated by androgens and postnatal epididymal development. METHODOLOGY/PRINCIPAL FINDINGS: Mice were deprived of circulating androgen by bilaterally castration and were then supplemented with exogenous testosterone propionate for one week. MTA1 was immunolocalized in the epithelium of the entire epididymis with the maximal expression in the nuclei of principal cells and of clear cells in proximal region. Its expression decreased gradually after castration, whereas testosterone treatment could restore the expression, indicating that the expression of this gene is dependent on androgen. During postnatal development, the protein expression in the epididymis began to appear from day 7 to day 14, increased dramatically from postnatal day 28, and peaked at adulthood onwards, coinciding with both the well differentiated status of epididymis and the mature levels of circulating androgens. This region- and cell-specific pattern was also conservative in normal human epididymis. CONCLUSIONS: Our data suggest that the expression of MTA1 protein could be regulated by androgen pathway and its expression level is closely associated with the postnatal development of the epididymis, giving rise to the possibility that this gene plays a potential role in sperm maturation and fertility. |
format | Text |
id | pubmed-2972736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29727362010-11-16 Localization and Androgen Regulation of Metastasis-Associated Protein 1 in Mouse Epididymis Ma, Li Li, Wei Zhu, Hua-Ping Li, Zhen Sun, Zhi-Jian Liu, Xin-Ping Zhao, Jie Zhang, Jin-Shan Zhang, Yuan-Qiang PLoS One Research Article BACKGROUND: Metastasis-associated protein 1 (MTA1), the founding member of the MTA family of genes, can modulate transcription by influencing the status of chromatin remodeling. Despite its strong correlation with the metastatic potential of cancer cells, MTA1 can also regulate crucial cellular pathways by modifying the acetylation status. We have previously reported the presence of MTA1/MTA1 in human and mouse testes, providing the evidence for its involvement in the regulation of testicular function during murine spermatogenesis. The objective of present study was to further assess the localization of MTA1 in mouse epididymis on both transcriptional and translational level, and then to explore whether MTA1 expression is regulated by androgens and postnatal epididymal development. METHODOLOGY/PRINCIPAL FINDINGS: Mice were deprived of circulating androgen by bilaterally castration and were then supplemented with exogenous testosterone propionate for one week. MTA1 was immunolocalized in the epithelium of the entire epididymis with the maximal expression in the nuclei of principal cells and of clear cells in proximal region. Its expression decreased gradually after castration, whereas testosterone treatment could restore the expression, indicating that the expression of this gene is dependent on androgen. During postnatal development, the protein expression in the epididymis began to appear from day 7 to day 14, increased dramatically from postnatal day 28, and peaked at adulthood onwards, coinciding with both the well differentiated status of epididymis and the mature levels of circulating androgens. This region- and cell-specific pattern was also conservative in normal human epididymis. CONCLUSIONS: Our data suggest that the expression of MTA1 protein could be regulated by androgen pathway and its expression level is closely associated with the postnatal development of the epididymis, giving rise to the possibility that this gene plays a potential role in sperm maturation and fertility. Public Library of Science 2010-11-03 /pmc/articles/PMC2972736/ /pubmed/21082030 http://dx.doi.org/10.1371/journal.pone.0015439 Text en Ma et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ma, Li Li, Wei Zhu, Hua-Ping Li, Zhen Sun, Zhi-Jian Liu, Xin-Ping Zhao, Jie Zhang, Jin-Shan Zhang, Yuan-Qiang Localization and Androgen Regulation of Metastasis-Associated Protein 1 in Mouse Epididymis |
title | Localization and Androgen Regulation of Metastasis-Associated Protein 1 in Mouse Epididymis |
title_full | Localization and Androgen Regulation of Metastasis-Associated Protein 1 in Mouse Epididymis |
title_fullStr | Localization and Androgen Regulation of Metastasis-Associated Protein 1 in Mouse Epididymis |
title_full_unstemmed | Localization and Androgen Regulation of Metastasis-Associated Protein 1 in Mouse Epididymis |
title_short | Localization and Androgen Regulation of Metastasis-Associated Protein 1 in Mouse Epididymis |
title_sort | localization and androgen regulation of metastasis-associated protein 1 in mouse epididymis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2972736/ https://www.ncbi.nlm.nih.gov/pubmed/21082030 http://dx.doi.org/10.1371/journal.pone.0015439 |
work_keys_str_mv | AT mali localizationandandrogenregulationofmetastasisassociatedprotein1inmouseepididymis AT liwei localizationandandrogenregulationofmetastasisassociatedprotein1inmouseepididymis AT zhuhuaping localizationandandrogenregulationofmetastasisassociatedprotein1inmouseepididymis AT lizhen localizationandandrogenregulationofmetastasisassociatedprotein1inmouseepididymis AT sunzhijian localizationandandrogenregulationofmetastasisassociatedprotein1inmouseepididymis AT liuxinping localizationandandrogenregulationofmetastasisassociatedprotein1inmouseepididymis AT zhaojie localizationandandrogenregulationofmetastasisassociatedprotein1inmouseepididymis AT zhangjinshan localizationandandrogenregulationofmetastasisassociatedprotein1inmouseepididymis AT zhangyuanqiang localizationandandrogenregulationofmetastasisassociatedprotein1inmouseepididymis |