Cargando…
Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells
BACKGROUND: Nasopharyngeal carcinoma (NPC) is a human epithelial malignancy consistently associated with the Epstein-Barr virus. The viral genome is contained in the nuclei of all malignant cells with abundant transcription of a family of viral microRNAs called BART miRNAs. MicroRNAs are well known...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974674/ https://www.ncbi.nlm.nih.gov/pubmed/20950422 http://dx.doi.org/10.1186/1743-422X-7-271 |
_version_ | 1782190889349677056 |
---|---|
author | Gourzones, Claire Gelin, Aurore Bombik, Izabela Klibi, Jihène Vérillaud, Benjamin Guigay, Joël Lang, Philippe Témam, Stéphane Schneider, Véronique Amiel, Corinne Baconnais, Sonia Jimenez, Anne-Sophie Busson, Pierre |
author_facet | Gourzones, Claire Gelin, Aurore Bombik, Izabela Klibi, Jihène Vérillaud, Benjamin Guigay, Joël Lang, Philippe Témam, Stéphane Schneider, Véronique Amiel, Corinne Baconnais, Sonia Jimenez, Anne-Sophie Busson, Pierre |
author_sort | Gourzones, Claire |
collection | PubMed |
description | BACKGROUND: Nasopharyngeal carcinoma (NPC) is a human epithelial malignancy consistently associated with the Epstein-Barr virus. The viral genome is contained in the nuclei of all malignant cells with abundant transcription of a family of viral microRNAs called BART miRNAs. MicroRNAs are well known intra-cellular regulatory elements of gene expression. In addition, they are often exported in the extra-cellular space and sometimes transferred in recipient cells distinct from the producer cells. Extra-cellular transport of the microRNAs is facilitated by various processes including association with protective proteins and packaging in secreted nanovesicles called exosomes. Presence of microRNAS produced by malignant cells has been reported in the blood and saliva of tumor-bearing patients, especially patients diagnosed with glioblastoma or ovarian carcinoma. In this context, it was decided to investigate extra-cellular release of BART miRNAs by NPC cells and their possible detection in the blood of NPC patients. To address this question, we investigated by quantitative RT-PCR the status of 5 microRNAs from the BART family in exosomes released by NPC cells in vitro as well as in plasma samples from NPC xenografted nude mice and NPC patients. RESULTS: We report that the BART miRNAs are released in the extra-cellular space by NPC cells being associated, at least to a large extent, with secreted exosomes. They are detected with a good selectivity in plasma samples from NPC xenografted nude mice as well as NPC patients. CONCLUSIONS: Viral BART miRNAs are secreted by NPC cells in vitro and in vivo. They have enough stability to diffuse from the tumor site to the peripheral blood. This study provides a basis to explore their potential as a source of novel tumor biomarkers and their possible role in communications between malignant and non-malignant cells. |
format | Text |
id | pubmed-2974674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29746742010-11-06 Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells Gourzones, Claire Gelin, Aurore Bombik, Izabela Klibi, Jihène Vérillaud, Benjamin Guigay, Joël Lang, Philippe Témam, Stéphane Schneider, Véronique Amiel, Corinne Baconnais, Sonia Jimenez, Anne-Sophie Busson, Pierre Virol J Research BACKGROUND: Nasopharyngeal carcinoma (NPC) is a human epithelial malignancy consistently associated with the Epstein-Barr virus. The viral genome is contained in the nuclei of all malignant cells with abundant transcription of a family of viral microRNAs called BART miRNAs. MicroRNAs are well known intra-cellular regulatory elements of gene expression. In addition, they are often exported in the extra-cellular space and sometimes transferred in recipient cells distinct from the producer cells. Extra-cellular transport of the microRNAs is facilitated by various processes including association with protective proteins and packaging in secreted nanovesicles called exosomes. Presence of microRNAS produced by malignant cells has been reported in the blood and saliva of tumor-bearing patients, especially patients diagnosed with glioblastoma or ovarian carcinoma. In this context, it was decided to investigate extra-cellular release of BART miRNAs by NPC cells and their possible detection in the blood of NPC patients. To address this question, we investigated by quantitative RT-PCR the status of 5 microRNAs from the BART family in exosomes released by NPC cells in vitro as well as in plasma samples from NPC xenografted nude mice and NPC patients. RESULTS: We report that the BART miRNAs are released in the extra-cellular space by NPC cells being associated, at least to a large extent, with secreted exosomes. They are detected with a good selectivity in plasma samples from NPC xenografted nude mice as well as NPC patients. CONCLUSIONS: Viral BART miRNAs are secreted by NPC cells in vitro and in vivo. They have enough stability to diffuse from the tumor site to the peripheral blood. This study provides a basis to explore their potential as a source of novel tumor biomarkers and their possible role in communications between malignant and non-malignant cells. BioMed Central 2010-10-15 /pmc/articles/PMC2974674/ /pubmed/20950422 http://dx.doi.org/10.1186/1743-422X-7-271 Text en Copyright ©2010 Gourzones et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Gourzones, Claire Gelin, Aurore Bombik, Izabela Klibi, Jihène Vérillaud, Benjamin Guigay, Joël Lang, Philippe Témam, Stéphane Schneider, Véronique Amiel, Corinne Baconnais, Sonia Jimenez, Anne-Sophie Busson, Pierre Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells |
title | Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells |
title_full | Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells |
title_fullStr | Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells |
title_full_unstemmed | Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells |
title_short | Extra-cellular release and blood diffusion of BART viral micro-RNAs produced by EBV-infected nasopharyngeal carcinoma cells |
title_sort | extra-cellular release and blood diffusion of bart viral micro-rnas produced by ebv-infected nasopharyngeal carcinoma cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974674/ https://www.ncbi.nlm.nih.gov/pubmed/20950422 http://dx.doi.org/10.1186/1743-422X-7-271 |
work_keys_str_mv | AT gourzonesclaire extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT gelinaurore extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT bombikizabela extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT klibijihene extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT verillaudbenjamin extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT guigayjoel extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT langphilippe extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT temamstephane extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT schneiderveronique extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT amielcorinne extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT baconnaissonia extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT jimenezannesophie extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells AT bussonpierre extracellularreleaseandblooddiffusionofbartviralmicrornasproducedbyebvinfectednasopharyngealcarcinomacells |