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Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response
Non-thyroidal illness is characterized by low tri-iodothyronine (T3) serum level under acute-phase conditions. We studied hepatic gene expression of the newly identified thyroid hormone receptor (TR) cofactor DOR/TP53INP2 together with TRs in a rat model of aseptic abscesses induced by injecting int...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Springer-Verlag
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977073/ https://www.ncbi.nlm.nih.gov/pubmed/20949361 http://dx.doi.org/10.1007/s00441-010-1067-4 |
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author | Malik, Ihtzaz Ahmed Baumgartner, Bernhard G. Naz, Naila Sheikh, Nadeem Moriconi, Federico Ramadori, Giuliano |
author_facet | Malik, Ihtzaz Ahmed Baumgartner, Bernhard G. Naz, Naila Sheikh, Nadeem Moriconi, Federico Ramadori, Giuliano |
author_sort | Malik, Ihtzaz Ahmed |
collection | PubMed |
description | Non-thyroidal illness is characterized by low tri-iodothyronine (T3) serum level under acute-phase conditions. We studied hepatic gene expression of the newly identified thyroid hormone receptor (TR) cofactor DOR/TP53INP2 together with TRs in a rat model of aseptic abscesses induced by injecting intramuscular turpentine-oil into each hind limb. A fast (4-6 h) decrease in the serum level of free thyroxine and free T3 was observed. By immunohistology, abundant DOR protein expression was detected in the nuclei of hepatocytes and ED-1(+) (mononuclear phagocytes), CK-19(+) (biliary cells), and SMA(+) (mesenchymal cells of the portal tract) cells. DOR signal was reduced with a minimum at 6-12 h after the acute-phase reaction (APR). Immunohistology also showed a similar pattern of protein expression in TRα1 but without a significant change during APR. Transcripts specific for DOR, nuclear receptor co-repressor 1 (NCoR-1), and TRβ1 were down-regulated with a minimum at 6-12 h, whereas expression for TRα1 and TRα2 was slightly and significantly up-regulated, respectively, with a maximum at 24 h after APR was initiated. In cultured hepatocytes, acute-phase cytokines interleukin-1β (IL-1β) and IL-6 down-regulated DOR and TRβ1 at the mRNA level. Moreover, gene expression of DOR and TRs (TRα1, TRα2, and TRβ1) was up-regulated in hepatocytes by adding T3 to the culture medium; this up-regulation was almost completely blocked by treating the cells with IL-6. Thus, TRβ1, NCoR-1, and the recently identified DOR/TP53INP2 are abundantly expressed and down-regulated in liver cells during APR. Their down-regulation is attributable to the decreased serum level of thyroid hormones and most probably also to the direct action of the main acute-phase cytokines. |
format | Text |
id | pubmed-2977073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-29770732010-12-15 Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response Malik, Ihtzaz Ahmed Baumgartner, Bernhard G. Naz, Naila Sheikh, Nadeem Moriconi, Federico Ramadori, Giuliano Cell Tissue Res Regular Article Non-thyroidal illness is characterized by low tri-iodothyronine (T3) serum level under acute-phase conditions. We studied hepatic gene expression of the newly identified thyroid hormone receptor (TR) cofactor DOR/TP53INP2 together with TRs in a rat model of aseptic abscesses induced by injecting intramuscular turpentine-oil into each hind limb. A fast (4-6 h) decrease in the serum level of free thyroxine and free T3 was observed. By immunohistology, abundant DOR protein expression was detected in the nuclei of hepatocytes and ED-1(+) (mononuclear phagocytes), CK-19(+) (biliary cells), and SMA(+) (mesenchymal cells of the portal tract) cells. DOR signal was reduced with a minimum at 6-12 h after the acute-phase reaction (APR). Immunohistology also showed a similar pattern of protein expression in TRα1 but without a significant change during APR. Transcripts specific for DOR, nuclear receptor co-repressor 1 (NCoR-1), and TRβ1 were down-regulated with a minimum at 6-12 h, whereas expression for TRα1 and TRα2 was slightly and significantly up-regulated, respectively, with a maximum at 24 h after APR was initiated. In cultured hepatocytes, acute-phase cytokines interleukin-1β (IL-1β) and IL-6 down-regulated DOR and TRβ1 at the mRNA level. Moreover, gene expression of DOR and TRs (TRα1, TRα2, and TRβ1) was up-regulated in hepatocytes by adding T3 to the culture medium; this up-regulation was almost completely blocked by treating the cells with IL-6. Thus, TRβ1, NCoR-1, and the recently identified DOR/TP53INP2 are abundantly expressed and down-regulated in liver cells during APR. Their down-regulation is attributable to the decreased serum level of thyroid hormones and most probably also to the direct action of the main acute-phase cytokines. Springer-Verlag 2010-10-15 2010 /pmc/articles/PMC2977073/ /pubmed/20949361 http://dx.doi.org/10.1007/s00441-010-1067-4 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Regular Article Malik, Ihtzaz Ahmed Baumgartner, Bernhard G. Naz, Naila Sheikh, Nadeem Moriconi, Federico Ramadori, Giuliano Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response |
title | Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response |
title_full | Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response |
title_fullStr | Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response |
title_full_unstemmed | Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response |
title_short | Changes in gene expression of DOR and other thyroid hormone receptors in rat liver during acute-phase response |
title_sort | changes in gene expression of dor and other thyroid hormone receptors in rat liver during acute-phase response |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2977073/ https://www.ncbi.nlm.nih.gov/pubmed/20949361 http://dx.doi.org/10.1007/s00441-010-1067-4 |
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