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Quantitative Assessment of the Sensitivity of Various Commercial Reverse Transcriptases Based on Armored HIV RNA

BACKGROUND: The in-vitro reverse transcription of RNA to its complementary DNA, catalyzed by the enzyme reverse transcriptase, is the most fundamental step in the quantitative RNA detection in genomic studies. As such, this step should be as analytically sensitive, efficient and reproducible as poss...

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Detalles Bibliográficos
Autores principales: Okello, John B. A., Rodriguez, Linda, Poinar, Debi, Bos, Kirsten, Okwi, Andrew L., Bimenya, Gabriel S., Sewankambo, Nelson K., Henry, Kenneth R., Kuch, Melanie, Poinar, Hendrik N.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978101/
https://www.ncbi.nlm.nih.gov/pubmed/21085668
http://dx.doi.org/10.1371/journal.pone.0013931