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Quantitative Assessment of the Sensitivity of Various Commercial Reverse Transcriptases Based on Armored HIV RNA
BACKGROUND: The in-vitro reverse transcription of RNA to its complementary DNA, catalyzed by the enzyme reverse transcriptase, is the most fundamental step in the quantitative RNA detection in genomic studies. As such, this step should be as analytically sensitive, efficient and reproducible as poss...
Autores principales: | Okello, John B. A., Rodriguez, Linda, Poinar, Debi, Bos, Kirsten, Okwi, Andrew L., Bimenya, Gabriel S., Sewankambo, Nelson K., Henry, Kenneth R., Kuch, Melanie, Poinar, Hendrik N. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978101/ https://www.ncbi.nlm.nih.gov/pubmed/21085668 http://dx.doi.org/10.1371/journal.pone.0013931 |
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