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Intravital spectral imaging as a tool for accurate measurement of vascularization in mice

BACKGROUND: Quantitative determination of the development of new blood vessels is crucial for our understanding of the progression of several diseases, including cancer. However, in most cases a high throughput technique that is simple, accurate, user-independent and cost-effective for small animal...

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Autores principales: Arranz, Alicia, Androulidaki, Ariadne, Mol, Berber, Tsentelierou, Eleftheria, Stathopoulos, Efstathios N, Tsatsanis, Christos, Ripoll, Jorge
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978126/
https://www.ncbi.nlm.nih.gov/pubmed/20974005
http://dx.doi.org/10.1186/2040-2384-2-22
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author Arranz, Alicia
Androulidaki, Ariadne
Mol, Berber
Tsentelierou, Eleftheria
Stathopoulos, Efstathios N
Tsatsanis, Christos
Ripoll, Jorge
author_facet Arranz, Alicia
Androulidaki, Ariadne
Mol, Berber
Tsentelierou, Eleftheria
Stathopoulos, Efstathios N
Tsatsanis, Christos
Ripoll, Jorge
author_sort Arranz, Alicia
collection PubMed
description BACKGROUND: Quantitative determination of the development of new blood vessels is crucial for our understanding of the progression of several diseases, including cancer. However, in most cases a high throughput technique that is simple, accurate, user-independent and cost-effective for small animal imaging is not available. METHODS: In this work we present a simple approach based on spectral imaging to increase the contrast between vessels and surrounding tissue, enabling accurate determination of the blood vessel area. This approach is put to test with a 4T1 breast cancer murine in vivo model and validated with histological and microvessel density analysis. RESULTS: We found that one can accurately measure the vascularization area by using excitation/emission filter pairs which enhance the surrounding tissue's autofluorescence, significantly increasing the contrast between surrounding tissue and blood vessels. Additionally, we found excellent correlation between this technique and histological and microvessel density analysis. CONCLUSIONS: Making use of spectral imaging techniques we have shown that it is possible to accurately determine blood vessel volume intra-vitally. We believe that due to the low cost, accuracy, user-independence and simplicity of this technique, it will be of great value in those cases where in vivo quantitative information is necessary.
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spelling pubmed-29781262010-11-11 Intravital spectral imaging as a tool for accurate measurement of vascularization in mice Arranz, Alicia Androulidaki, Ariadne Mol, Berber Tsentelierou, Eleftheria Stathopoulos, Efstathios N Tsatsanis, Christos Ripoll, Jorge J Angiogenes Res Methodology BACKGROUND: Quantitative determination of the development of new blood vessels is crucial for our understanding of the progression of several diseases, including cancer. However, in most cases a high throughput technique that is simple, accurate, user-independent and cost-effective for small animal imaging is not available. METHODS: In this work we present a simple approach based on spectral imaging to increase the contrast between vessels and surrounding tissue, enabling accurate determination of the blood vessel area. This approach is put to test with a 4T1 breast cancer murine in vivo model and validated with histological and microvessel density analysis. RESULTS: We found that one can accurately measure the vascularization area by using excitation/emission filter pairs which enhance the surrounding tissue's autofluorescence, significantly increasing the contrast between surrounding tissue and blood vessels. Additionally, we found excellent correlation between this technique and histological and microvessel density analysis. CONCLUSIONS: Making use of spectral imaging techniques we have shown that it is possible to accurately determine blood vessel volume intra-vitally. We believe that due to the low cost, accuracy, user-independence and simplicity of this technique, it will be of great value in those cases where in vivo quantitative information is necessary. BioMed Central 2010-10-25 /pmc/articles/PMC2978126/ /pubmed/20974005 http://dx.doi.org/10.1186/2040-2384-2-22 Text en Copyright ©2010 Arranz et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Arranz, Alicia
Androulidaki, Ariadne
Mol, Berber
Tsentelierou, Eleftheria
Stathopoulos, Efstathios N
Tsatsanis, Christos
Ripoll, Jorge
Intravital spectral imaging as a tool for accurate measurement of vascularization in mice
title Intravital spectral imaging as a tool for accurate measurement of vascularization in mice
title_full Intravital spectral imaging as a tool for accurate measurement of vascularization in mice
title_fullStr Intravital spectral imaging as a tool for accurate measurement of vascularization in mice
title_full_unstemmed Intravital spectral imaging as a tool for accurate measurement of vascularization in mice
title_short Intravital spectral imaging as a tool for accurate measurement of vascularization in mice
title_sort intravital spectral imaging as a tool for accurate measurement of vascularization in mice
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978126/
https://www.ncbi.nlm.nih.gov/pubmed/20974005
http://dx.doi.org/10.1186/2040-2384-2-22
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