Cargando…
Characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice
The neuropeptide galanin is widely expressed in both the central and peripheral nervous systems and is involved in many diverse biological functions. There is a substantial data set that demonstrates galanin is upregulated after injury in the DRG, spinal cord and in many brain regions where it plays...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978139/ https://www.ncbi.nlm.nih.gov/pubmed/20964829 http://dx.doi.org/10.1186/1744-8069-6-67 |
_version_ | 1782191222354345984 |
---|---|
author | Pope, Robert JP Holmes, Fiona E Kerr, Niall C Wynick, David |
author_facet | Pope, Robert JP Holmes, Fiona E Kerr, Niall C Wynick, David |
author_sort | Pope, Robert JP |
collection | PubMed |
description | The neuropeptide galanin is widely expressed in both the central and peripheral nervous systems and is involved in many diverse biological functions. There is a substantial data set that demonstrates galanin is upregulated after injury in the DRG, spinal cord and in many brain regions where it plays a predominantly antinociceptive role in addition to being neuroprotective and pro-regenerative. To further characterise the role of galanin following nerve injury, a novel transgenic line was created using the binary transgenic tet-off system, to overexpress galanin in galaninergic tissue in a suppressible manner. The double transgenic mice express significantly more galanin in the DRG one week after sciatic nerve section (axotomy) compared to WT mice and this overexpression is suppressible upon administration of doxycycline. Phenotypic analysis revealed markedly attenuated allodynia when galanin is overexpressed and an increase in allodynia following galanin suppression. This novel transgenic line demonstrates that whether galanin expression is increased at the time of nerve injury or only after allodynia is established, the neuropeptide is able to reduce neuropathic pain behaviour. These new findings imply that administration of a galanin agonist to patients with established allodynia would be an effective treatment for neuropathic pain. |
format | Text |
id | pubmed-2978139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29781392010-11-11 Characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice Pope, Robert JP Holmes, Fiona E Kerr, Niall C Wynick, David Mol Pain Short Report The neuropeptide galanin is widely expressed in both the central and peripheral nervous systems and is involved in many diverse biological functions. There is a substantial data set that demonstrates galanin is upregulated after injury in the DRG, spinal cord and in many brain regions where it plays a predominantly antinociceptive role in addition to being neuroprotective and pro-regenerative. To further characterise the role of galanin following nerve injury, a novel transgenic line was created using the binary transgenic tet-off system, to overexpress galanin in galaninergic tissue in a suppressible manner. The double transgenic mice express significantly more galanin in the DRG one week after sciatic nerve section (axotomy) compared to WT mice and this overexpression is suppressible upon administration of doxycycline. Phenotypic analysis revealed markedly attenuated allodynia when galanin is overexpressed and an increase in allodynia following galanin suppression. This novel transgenic line demonstrates that whether galanin expression is increased at the time of nerve injury or only after allodynia is established, the neuropeptide is able to reduce neuropathic pain behaviour. These new findings imply that administration of a galanin agonist to patients with established allodynia would be an effective treatment for neuropathic pain. BioMed Central 2010-10-21 /pmc/articles/PMC2978139/ /pubmed/20964829 http://dx.doi.org/10.1186/1744-8069-6-67 Text en Copyright ©2010 Pope et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Pope, Robert JP Holmes, Fiona E Kerr, Niall C Wynick, David Characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice |
title | Characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice |
title_full | Characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice |
title_fullStr | Characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice |
title_full_unstemmed | Characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice |
title_short | Characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice |
title_sort | characterisation of the nociceptive phenotype of suppressible galanin overexpressing transgenic mice |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978139/ https://www.ncbi.nlm.nih.gov/pubmed/20964829 http://dx.doi.org/10.1186/1744-8069-6-67 |
work_keys_str_mv | AT poperobertjp characterisationofthenociceptivephenotypeofsuppressiblegalaninoverexpressingtransgenicmice AT holmesfionae characterisationofthenociceptivephenotypeofsuppressiblegalaninoverexpressingtransgenicmice AT kerrniallc characterisationofthenociceptivephenotypeofsuppressiblegalaninoverexpressingtransgenicmice AT wynickdavid characterisationofthenociceptivephenotypeofsuppressiblegalaninoverexpressingtransgenicmice |