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BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism
The GGCC-specific restriction endonuclease BspRI is one of the few Type IIP restriction endonucleases, which were suggested to be a monomer. Amino acid sequence information obtained by Edman sequencing and mass spectrometry analysis was used to clone the gene encoding BspRI. The bspRIR gene is locat...
| Autores principales: | , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978348/ https://www.ncbi.nlm.nih.gov/pubmed/20587501 http://dx.doi.org/10.1093/nar/gkq567 |