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A yeast one-hybrid system to screen for methylated DNA-binding proteins

We had previously exploited a method for targeted DNA methylation in budding yeast to succeed in one-hybrid detection of methylation-dependent DNA–protein interactions. Based on this finding, we developed a yeast one-hybrid system to screen cDNA libraries for clones encoding methylated DNA-binding p...

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Detalles Bibliográficos
Autores principales: Feng, Shu-Ying, Ota, Kazuhisa, Ito, Takashi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978385/
https://www.ncbi.nlm.nih.gov/pubmed/20798175
http://dx.doi.org/10.1093/nar/gkq757