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Structure of a Switchable Subtilisin Complexed with a Substrate and with the Activator Azide
[Image: see text] An engineered variant of the protease subtilisin from Bacillus amyloliquefaciens, in which the D32A mutation renders the enzyme’s activity dependent on the presence of certain small anions such as fluoride or azide, has been produced. This modified enzyme has applications as an azi...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2979009/ https://www.ncbi.nlm.nih.gov/pubmed/19761257 http://dx.doi.org/10.1021/bi900577n |