BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei

A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of...

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Autores principales: Bowers, Jolene R., Engelthaler, David M., Ginther, Jennifer L., Pearson, Talima, Peacock, Sharon J., Tuanyok, Apichai, Wagner, David M., Currie, Bart J., Keim, Paul S.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2980470/
https://www.ncbi.nlm.nih.gov/pubmed/21103048
http://dx.doi.org/10.1371/journal.pone.0015413
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author Bowers, Jolene R.
Engelthaler, David M.
Ginther, Jennifer L.
Pearson, Talima
Peacock, Sharon J.
Tuanyok, Apichai
Wagner, David M.
Currie, Bart J.
Keim, Paul S.
author_facet Bowers, Jolene R.
Engelthaler, David M.
Ginther, Jennifer L.
Pearson, Talima
Peacock, Sharon J.
Tuanyok, Apichai
Wagner, David M.
Currie, Bart J.
Keim, Paul S.
author_sort Bowers, Jolene R.
collection PubMed
description A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei.
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spelling pubmed-29804702010-11-22 BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei Bowers, Jolene R. Engelthaler, David M. Ginther, Jennifer L. Pearson, Talima Peacock, Sharon J. Tuanyok, Apichai Wagner, David M. Currie, Bart J. Keim, Paul S. PLoS One Research Article A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei. Public Library of Science 2010-11-12 /pmc/articles/PMC2980470/ /pubmed/21103048 http://dx.doi.org/10.1371/journal.pone.0015413 Text en Bowers et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bowers, Jolene R.
Engelthaler, David M.
Ginther, Jennifer L.
Pearson, Talima
Peacock, Sharon J.
Tuanyok, Apichai
Wagner, David M.
Currie, Bart J.
Keim, Paul S.
BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei
title BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei
title_full BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei
title_fullStr BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei
title_full_unstemmed BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei
title_short BurkDiff: A Real-Time PCR Allelic Discrimination Assay for Burkholderia Pseudomallei and B. mallei
title_sort burkdiff: a real-time pcr allelic discrimination assay for burkholderia pseudomallei and b. mallei
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2980470/
https://www.ncbi.nlm.nih.gov/pubmed/21103048
http://dx.doi.org/10.1371/journal.pone.0015413
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