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Charting the Secretory Pathway in a Simple Eukaryote
George Palade, a founding father of cell biology and of the American Society for Cell Biology (ASCB), established the ultrastructural framework for an analysis of how proteins are secreted and membranes are assembled in eukaryotic cells. His vision inspired a generation of investigators to probe the...
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Formato: | Texto |
Lenguaje: | English |
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The American Society for Cell Biology
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2982102/ https://www.ncbi.nlm.nih.gov/pubmed/21079008 http://dx.doi.org/10.1091/mbc.E10-05-0416 |
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author | Schekman, Randy |
author_facet | Schekman, Randy |
author_sort | Schekman, Randy |
collection | PubMed |
description | George Palade, a founding father of cell biology and of the American Society for Cell Biology (ASCB), established the ultrastructural framework for an analysis of how proteins are secreted and membranes are assembled in eukaryotic cells. His vision inspired a generation of investigators to probe the molecular mechanisms of protein transport. My laboratory has dissected these pathways with complementary genetic and biochemical approaches. Peter Novick, one of my first graduate students, isolated secretion mutants of Saccharomyces cerevisiae, and through cytological analysis of single and double mutants and molecular cloning of the corresponding SEC genes, we established that yeast cells use a secretory pathway fundamentally conserved in all eukaryotes. A biochemical reaction that recapitulates the first half of the secretory pathway was used to characterize Sec proteins that comprise the polypeptide translocation channel in the endoplasmic reticulum (ER) membrane (Sec61) and the cytoplasmic coat protein complex (COPII) that captures cargo proteins into transport vesicles that bud from the ER. |
format | Text |
id | pubmed-2982102 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-29821022011-01-30 Charting the Secretory Pathway in a Simple Eukaryote Schekman, Randy Mol Biol Cell ASCB Award Essay George Palade, a founding father of cell biology and of the American Society for Cell Biology (ASCB), established the ultrastructural framework for an analysis of how proteins are secreted and membranes are assembled in eukaryotic cells. His vision inspired a generation of investigators to probe the molecular mechanisms of protein transport. My laboratory has dissected these pathways with complementary genetic and biochemical approaches. Peter Novick, one of my first graduate students, isolated secretion mutants of Saccharomyces cerevisiae, and through cytological analysis of single and double mutants and molecular cloning of the corresponding SEC genes, we established that yeast cells use a secretory pathway fundamentally conserved in all eukaryotes. A biochemical reaction that recapitulates the first half of the secretory pathway was used to characterize Sec proteins that comprise the polypeptide translocation channel in the endoplasmic reticulum (ER) membrane (Sec61) and the cytoplasmic coat protein complex (COPII) that captures cargo proteins into transport vesicles that bud from the ER. The American Society for Cell Biology 2010-11-15 /pmc/articles/PMC2982102/ /pubmed/21079008 http://dx.doi.org/10.1091/mbc.E10-05-0416 Text en © 2010 by The American Society for Cell Biology This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). |
spellingShingle | ASCB Award Essay Schekman, Randy Charting the Secretory Pathway in a Simple Eukaryote |
title | Charting the Secretory Pathway in a Simple Eukaryote |
title_full | Charting the Secretory Pathway in a Simple Eukaryote |
title_fullStr | Charting the Secretory Pathway in a Simple Eukaryote |
title_full_unstemmed | Charting the Secretory Pathway in a Simple Eukaryote |
title_short | Charting the Secretory Pathway in a Simple Eukaryote |
title_sort | charting the secretory pathway in a simple eukaryote |
topic | ASCB Award Essay |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2982102/ https://www.ncbi.nlm.nih.gov/pubmed/21079008 http://dx.doi.org/10.1091/mbc.E10-05-0416 |
work_keys_str_mv | AT schekmanrandy chartingthesecretorypathwayinasimpleeukaryote |